Why does the fatigue cracks initiate from surface to interior as the stress level decreases?

It is well known that low-cycle and high-cycle fatigue cracks initiate from the surface, while ultra-high cycle fatigue cracks initiate from the inside. I am very curious why this transformation...

17 January 2021 9,633 6 View

How to understand the chemo-mechanical coupling?

Recently, I came into contact with a new discipline named chemo-mechanical coupling. I found that chemo-mechanical coupling phenomena exist in many research areas, ranging from development of...

17 December 2020 1,065 3 View

I am working on membrane distillation model in COMSOL and I have some problems coming in it?

I need to add a latent heat flux of evaporation to the hot side boundary, which is a function of the pressure difference between the cold side and the hot side boundary.However, the pressure at...

16 December 2020 9,890 1 View

How to spin coat thin film for PEC devices (improve viscosity)?

I am trying to repeat the fabrication of BiVO4 (BVO) using the metal-organic deposition method. This is a well-reported way to make BVO thin film on FTO. So basically, I just dissolve Bi(NO3)3...

18 March 2020 8,405 7 View

Why ecology system allows virus exsit?

27 February 2020 5,293 3 View

Reliable PGP9.5 antibody to visualize intraepidermal nerve fibres in rat paw skin?

Hi, I'm looking for a reliable PGP9.5 antibody that can visualize INEF in rat paw skin. Most publications with good image on rat paw uses Ultraclone or AbD Serotec's rabbit anti-human antibody...

19 February 2020 979 2 View

Differential Scanning Calorimetry (DSC) protein aggregation?

I am working on Differential Scanning Calorimetry (DSC) for my protein. Mainly I want to check its melting temperature and enthalpy changes. I first tried two standard proteins ( BSA and...

17 February 2020 1,733 3 View

Why does DAB staining not work, given that TSA on the same tissue works?

I am staining for c-fos in ferret brain sections using this antibody (https://www.cellsignal.com/products/primary-antibodies/c-fos-9f6-rabbit-mab/2250). The sequence of ferret c-fos is highly...

09 January 2020 4,671 1 View

What gas flow rate should I choose for calcinating CaCO3 in the air ?

A lot of papers talk about calcination temperature, but little of them mention the gas flow rate. I find gas flow has a strong influence of my catalyst... I am preparing an organic Calcium...

18 November 2019 2,879 1 View

How to perform GC manual headspace sampling?

Hi everyone. May I know is there an established method for GC-FID headspace sampling? I did triplicates for the same concentration of analyte via manual sampling technique (using a air-tight gas...

17 July 2019 3,428 3 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

Do head direction cells necessarily encode in an allocentric reference frame?

According to the vertebrate literature, I got the impression that head-direction cells are always anchored to an allocentric reference frame. This can easily be tested with freely moving animals....

27 January 2021 3,821 3 View

Can you apply graph theoratic functional connectivity analysis to study cognitive processes under external stimuli?

Can functional connectivity analysis using Graph Theory (Hub-ness, clustering coefficient, etc) be applied to study cognitive processes? I felt most of the analysis to study the brain after...

25 January 2021 4,575 3 View

My Xenopus oocytes (healthy frogs) explode before I can make any electrophysiological records, why?

Xenopus laevis oocytes are used in electrophysiology to make transient expression of injected mRNA fragments. Through the technique of 2-electrodes voltage-clamp we can record the physiological...

11 January 2021 6,005 4 View

Excitatory / inhibitory ratio caculation ?

Hello everyone, Is it possible to calculate the E/I ratio on single-cell levels only based on miniature signals (Excitatory and inhibitory)? If yes, then what parameters should I take to...

01 December 2020 9,715 3 View

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29 November 2020 544 12 View

What is the best dye to use to visualise electroporation of neurons with DNA for later expression of a genetically-encoded fluorescent reporter?

We would like to electroporate neurons in organotypic slice cultures with DNA for genetically-encoded flourescent reporters (pH reporters, GEVIs etc). We would like to use an anionic dye that can...

17 November 2020 2,794 1 View

How to distinguish CA1 pyramidal cell vs. interneuron using voltage-clamp?

I am doing voltage-clamp experiments in CA1 pyramidal cells (mEPSCs; AMPA-NMDA ratio; LTP; etc) from acute hippocampal slices in mice. I want to make sure I am only recording from excitatory...

29 October 2020 1,098 2 View

What is the best anatomic plane to study BNST neurons using ex vivo slice culture in a rat?

I am looking to study neurons of the bed nucleus of the stria terminalis. I have seen reports using coronal sections. Others present the distinct nuclei in transverse sections. What is the best...

22 October 2020 3,343 1 View

No sodium current or action potentials during patch-clamping?

06 July 2020 6,409 5 View