I do not have experience on MEA (multi electrode array ) and I am looking for an in vitro slice assay being able to document pharmacological activities of PAM acting on the alpha-7 nicotinic ACh receptors in rat hippocampus ....does anyone have an idea how to do this?
Dear Aurel Sava, I believe the paper from Huang et al., published in Psychopharmacology (Berl). 2014 May;231(10):2199-210. doi: 10.1007/s00213-013-3373-5, may be useful for you, especially in the material and methods sections. I hope that this may help you.
Regards,
Wilson C.Santos
With no details about the precise targets of your study, detailed advices cannot be provided. However, if you want to evaluate in vivo effects, probably the best option is to use selective agonists (e.g. PHA543613, WAY317538, AR-R17779) or antagonists (e.g., alpha-conotoxin ArIB, α-Bungarotoxin) by means of microinjections to hippocampus. Then you can obtain neurochemical, electrophysiological or behavioral measures by means of a variety of methods. I hope this has been helpful...
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