I have encountered problems to calculate the concentration of Crocin in the natural saffron sample. Briefly, crocin is extracted from the samples (0.1 g to10 ml (0.80% MeOH), Shake in dark for an hour, Centrifuges(4000 rpm/15 min) and determined via the standard HPLC method. In each case, the sample peak area is compared with the standard (SIGMA 17304-1G), but the results show a total percentage of crocin higher than 100.
Hi.
You can calculate the concentration of crocin in your extract by using area relation between it and the standard (providing the same injection volume and analytical conditions are being used). Ideally prepare a calibration curve to quantify and run spiked samples to ensure your analytical system is not affected by interference.
Once you have a reliable extract concentration value, calculate as follows:
Mass of crocin in the extract = concentration of crocin in the extract x total volume of the extract.
You know that all the mass of crocin came from 0.1 g of sample, so just divide the mass you calculated by 0.1. Make sure that you're using the same units for the mass.
If you're doing it like this, and you still have a problem, then you must provide more details about the analytical you are using to get appropriate help.
Cheers
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