HPLC-DAD stands for High-Performance Liquid Chromatography with Diode Array Detection. It is a powerful analytical technique used to separate, identify, and quantify components in a mixture.
HPLC (High-Performance Liquid Chromatography): A technique used to separate compounds in a liquid sample by passing it through a column packed with a stationary phase. Different compounds are separated based on their interactions with the stationary phase and their solubility in the mobile phase.
DAD (Diode Array Detector): A type of detector in HPLC that monitors the absorption of ultraviolet (UV) and visible light across a range of wavelengths simultaneously. This allows for the detection and analysis of compounds based on their specific UV-Vis absorption spectra, providing more detailed information than single-wavelength detectors.
Together, HPLC-DAD is often used in pharmaceutical, environmental, and food industries for analyzing complex mixtures of chemicals. It can be especially useful for identifying compounds with unique UV-Vis spectra.But as usual sugars, and carbohydrates and oligosaccharides are not having conjugated double bonds thus HPLC-DAD is not much useful for glycans.
With both DAD and classical UV detectors you can do fructose and glucose, for example at 195 nm using acetonitrile/water as the mobile phase and a Phenomenex Luna Omerga Sugar column.
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