thank you
I have tried to use this kit to measure released somatostatin from isolated mouse islets, but I had an issue where all measurements were low which should not be the case. doe any body have any...
24 June 2024 1,552 0 View
If any one did include any consent questions (few lines) in a tenant satisfaction survey. For example, we use de identified data from different housing providers tenants for benchmarking purposes....
19 May 2024 724 0 View
There is a lot of matrix effect that disable me to quantify it. what i need is a quick and fast extraction method. what should i add?
13 May 2024 6,902 2 View
How to prepare the sections and how to immunostain with three different antibodies? As I am going to use antibody to stain receptor of interest and anti insulin and antiglucagon. How can the three...
28 December 2023 7,154 0 View
Hi , I am looking for a full protocol for immunostaining of mouse pancreatic islets after isolation. I put them in RMPI after isolation. How can I prepare them for staining? and can I do...
05 October 2023 9,326 2 View
portable water quality assessment of some selected sachet waters pdf?
22 September 2023 1,744 2 View
I am going to do a radioligand competition binding assay, could any one help me with a protocol. The Kd value of my radioligand is 6.4. And the final volume of the assay is 500microliter, do I...
22 August 2023 2,236 0 View
Which method or modern technology, as a science instructor, will help raise the academic performance of students in secondary school? An innovative approach to teaching of abstract concepts...
20 July 2023 1,715 8 View
what is the cause of high scintillation counts in 35s GTP gamma s binding assay, despite of optimizing ligand concentration and all other parameters.
27 June 2023 1,234 0 View
How can preparation of sample for western blotting from isolated pancreatic islet be done?
23 June 2023 7,871 0 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...
05 August 2024 3,246 1 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,033 1 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View
I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...
28 July 2024 3,559 0 View
I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...
23 July 2024 3,891 0 View
through genetic modification or metabolic engineering? Is it helpful, good for health ?
22 July 2024 7,811 4 View
Sulfuric acid has changed color to yellow.
16 July 2024 6,964 0 View
Hi, Attempted to extract cfDNA from a 4 mL plasma sample, with poor yield. Went through the manual in the section regarding poor yield, and feel that this does not apply. Some potential...
14 July 2024 7,475 1 View
Hi all, I am conducting research on screening peptides (20-35 amino acids) with potential for treating dengue fever through protein-peptide docking methods. In the protein database, I found the...
10 July 2024 8,037 1 View
