Anthony Ayodeji Adegoke
Growth of Pseudomonas aeruginosa: P . aeruginosa can still grow on cetrimide agar due to its metabolic flexibility. It may utilize other trace carbon sources present in the medium. Pigment Production:Pyocyanin and fluorescein production might be reduced or absent since glycerol acts as a key precursor for pigment synthesis. This could impact the visual identification of the organism. Selectivity: The agar remains selective for P. aeruginosa because cetrimide inhibits most other bacterial species. Growth in the presence of cetrimide is a strong presumptive indication of P. aeruginosa. You may simply perform additional biochemical tests (e.g., oxidase test, growth at 42°C, and ability to reduce nitrate).
YES
Cetrimide agar is specifically designed to isolate and identify Pseudomonas aeruginosa based on its ability to grow in the presence of cetrimide, a quaternary ammonium compound that inhibits the growth of most other bacteria. While glycerol is a recommended carbon source for optimal growth and pigment production (pyocyanin and fluorescein) in P. aeruginosa, the absence of glycerol does not necessarily prevent the detection of this organism. Here's what happens in such cases:
Without Glycerol:
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Zeyad K. Hussein
There is no effect of adding glycerol to cetrimide media to detect pseudomonus aeruginosa but increase selectivity of media to isolation of pseudomonus may be adding nalidixic acid antibiotic to media
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