Can anyone tell me about the heat sensitivity of aqueous solution of AgNo3? I have to use AgNo3 in MS media as ethylene inhibitor but a little bit confused how to use it should I autoclave it with media or add after autoclaving?
Thnx for the help sir, can u plz send me some link or paper which proves this..
Dear Yamini,
As advices by Mr. Singh , You have to add silver nitrate to the MS after autoclave and when it cools just before you pour the solution to plates. You can leave the magnetic stirrer inside the bottle for autoclave and add the solution to stir the media. As for the prep of silver nitrate solution, you can filter sterilize using millipore syringe. Hope this helps.
The issue with silver nitrate in MS salts really isn't heat sensitiviey at all. It is a question of solubility. I think you will have difficulty knowing what the actual concentration of Ag is in your solution. MS salts has both significant chloride and phosphate levels, and thus AgCl2 and Ag3(PO4)2 percipitates will form depending on pH of the medium. Therefore you will likely have a very low silver concentration in the medium, which may or may not be effective regardless of when in the media prep process silver is added to MS salts.
thank you Bob Locy but problem is not solubility or precipitation
silver nitrate is very much soluble in basal MS media n doesn't form any precipitation
except it doesnt gives the good results as when used without autoclaving and changes color when exposed to light
There's no need to keep ur cultures in dark as light is an important factor for growth of explant
And light doesnt affect little amount of AgNO3 present in media
I kept all my cultures in light and Got results as expected
But just for comparison u can keep control in dark
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