Have you tried any annealing of the powders and study the properties?

Why my gel electrophoresis has smearing?

Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...

22 July 2024 9,761 5 View

In scf input file if celldm(1) is used in bohrs unit,inorder to consistency of units is atomic position needs to be in crystal?

here is scf file &CONTROL title = 'cal' calculation = 'scf' etot_conv_thr = 4e-6 forc_conv_thr = 1e-6 pseudo_dir='./' !tprnfor = .true. !tstress =...

29 June 2024 10,082 0 View

How we can check the effect of light on magnetization by VAPS?

28 June 2024 9,366 0 View

What is the reason for the non-monotonic variation of roughness for sputtered Cu films annealed at 100, 200 and 300 degree Celsius?

I have prepared Copper thin films by magnetron sputtering and the samples have been annealed at 100, 200 and 300 degree celsius. The variation of the rms roughness and all other AFM height related...

11 June 2024 9,774 1 View

What is the ground state configuration of 2D-isotropy Heisenberg model at low temperature under classical MC method?

The Mermin-Wagner theorem has pointed out that there should be no spontaneous magnetization at finite temperature in 2D-isotropic Heisenberg model, or there will be no long-range ferromagnetic...

16 May 2024 7,070 2 View

Why am I getting a visible band in my negative control(which contains only nuclease-free water and no DNA template) in PCR?

I am doing my undergrad thesis that requires me to do PCR-RFLP analysis on DNA that I extracted from some blood samples. Initially, I was using the Qiagen mastermix for the optimization of my...

14 May 2024 4,370 4 View

How can I perform constrained SDFT of a material e.g fcc Ni? Can someone tell me what syntax should I give in my input files?

Recently I am studying on Exchange interaction and Magnetic force theorem(MFT) .. there the basic idea of MFT is we have to rotate the exchange-correlational (xc) potential by assuming that within...

29 April 2024 8,568 0 View

Any thoughts on troubleshooting my CC9 KO experiment?

Hi, I'm currently trying to generate a one-vector plasmid system for a knockout using CRISPR/Cas9. I'm using lentiCRISPRv2 (ampicillin-resistant) and following the GeCKO Target Guide Sequence...

29 April 2024 3,660 0 View

Regarding primers annealing temperature ?

I recently designed the qrtpcr primers from the 3'utr of the plant's genomic DNA keeping in mind that the annealing temperature should be 60 degree. Once I received the primers first to optimise...

25 April 2024 5,935 3 View

Why is there no Ligase in Ligation Independent Cloning?

Ligation Independent Cloning literally says no need Ligation, but it still needs ligase to anneal the fragment to vector. And the Protocol used T4 DNA Ligase for anneal process. What does this...

18 April 2024 1,494 3 View