I'm interested in the quality of the image. If so, can you share your images? Alternatively, do you have a relatively inexpensive setup (less than $5k) to image forams (including software) and pictures to share?
Dear Serena Dameron,
No matter which light microscope is done (depends only to the quality of the lens), it is necessary to process the images with the HeliconFocus program due to the thickness of the images and the difference of the focused area.
Therefore, a microscope equipped with a micro focus screw (fine adjustment) is required to produce the following stacks to building the final photo.
A foraminifer (Cornuspira foliacea (Philippi, 1844)) photograph which created with this method is attached.
Best wishes,
So this is used this with a canon/nikon camera, and I assume you use an adapter on your microscope to mount the camera? The image is beautiful!
No, this was taken with the DCM 500 microscope camera. I think the final image was obtained by combining a 45-50 stack. The most important element is lighting, it is also custom made with 5 gooseneck USB led. For diffuse light (to avoiding the light spots on the object) a printer paper barrier was placed between the specimen and the source of light. Microscope: trinocular light; Objective: Nikon 4/0.1 P; the camera was used with an extension for enlarging by attaching the camera to the trinocular port of the head.
I tried Dino-lite microscope once, but it was not successful. One reason is its low magnification, two is difficulty in focusing (it is too light and unstable to control). I recommend to find cheap but robust microscope (used, second hand one should be enough), attach ccd digital camera on it, and take pictures as Dr. Artüz did.
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