The original title is "Optimized Protocol of Chicken Antibody (IgY) Purification Providing Electrophoretically Homogenous Preparations ". I'm very curious about this article and I hope that someone in this site has already used it for other study.
In a personal letter, the student wrote me a question. I am particularly interested in exploring the application of UV-Vis spectroscopy as a method for assessing drug solubility in oils which...
08 April 2024 3,898 6 View
As we all know, everyone who studies physics is viewed as crazy mad scientists, I live this everyday with my family, jokes aside, how can we make the teaching of advanced physics subjects such as...
28 March 2024 7,172 10 View
Dear researchers, I am interested in the purification process of polyclonal antibodies for use in lateral flow immunoassays. Specifically, I would like to inquire about the common practices and...
14 February 2024 9,120 2 View
Dear colleague/friend As part of the thesis of the master candidate Sokaina Chroukate, we are exploring the concept of product circularity indicators and their applicability. We are reaching out...
04 February 2024 8,199 0 View
Hello, Looking at the published works the amount of IrCl3 used for the electrodeposition solution is extremely high, in many cases exceeding 1g per 50mL of solution and the Ti area is omitted...
31 October 2023 6,160 0 View
Hi All I would like to get some suggestions on how to store/preserve fresh invertebrate fecal samples in harsh conditions (dry and hot environment). I will be on the field for many days, possibly,...
18 January 2023 8,552 1 View
The intention is to produce polyclonal IgY antibodies.
25 August 2022 760 2 View
In a recent work, https://www.researchgate.net/publication/354541119_Drop_of_Solar_Wind_at_the_End_of_the_20th_Century we showed that the average parameters of the solar wind in the last 2 solar...
22 December 2021 320 8 View
Dear colleagues, maybe our team missed some important thing, but in two our works we have some results that look controversal. The first one includes the study, where we probed a PM fiber using...
05 December 2021 5,869 2 View
Dear community, I am currently modelling the hydrogen reduction of the iron ore pellet in COMSOL. Does anybody know how to assign reaction to a particular geometry? And how to obtain a reaction...
15 September 2021 1,574 0 View
My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...
07 August 2024 4,600 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
Purification of gst tagged protein
25 July 2024 1,720 0 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
Using INTRON's DNA-spin Plasmid DNA purification kit to extract plasmids from specific E.Coli strains, my team and I have encountered numerous plasmid dimerizations that lead to over-weighted...
23 July 2024 3,490 6 View
What is the heating temperature that I can use to deactivate enzymes in bacterial extract exopolysaccharide without loss of the bioactivity of EPS? Hi I am trying to make the purification (by TCA...
22 July 2024 5,334 6 View
Good day everyone, Trying to perform protein purification for the first time, gotta buy the whole equipment/reagents. Protein of interest 48 kDa (Antithrombin), expressed from his-tagged plasmid...
16 July 2024 3,692 6 View
Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...
13 July 2024 9,950 3 View