Arthropods metabarcoding: is there a storage method that would limit both contamination and DNA degradation?

I need to perform barcoding on a subset of specimen from arthropod bulk samples. As I want to be able to detect endoparasitoids as well, I am considering freezing my samples instead of storing...

04 August 2019 4,748 2 View

How to prepare fish (whole) in order to send it for analysis?

Hi, I have to collect fish, blend it, dry it and send it to a laboratory in order to have analysis of the fish composition. I need a whole fish, and I was wondering if there is a method to...

25 June 2019 1,884 2 View

Importance of light chain type on isotype control antibodies for flow cytometry?

I am putting together a panel for flow cytometry, for one particular antibody the only available isotype control has a different light chain to the test antibody. My test antibody is BV711-CD25...

10 June 2019 3,466 2 View

Optimising RT-qPCR for the detection of very low abundace IL-17A transcripts from human PBMC mRNA?

I am performing qPCR to quantify cytokine expression in human peripheral blood mononuclear cells. I have found it very difficult to get consistent results when assaying for IL-17A. Currently I am...

23 January 2019 8,404 1 View

Can DNase treatments to RNA preparations cause damage or loss to the mRNA?

When performing qPCR, I have noticed that cDNA made from RNA preparations treated with Turbo DNase (Invitrogen) prior to reverse transcription produce CT values that are shifted several cycles...

31 October 2018 9,688 5 View

What statistical test can I use to compare several distributions altogether?

I have 4 groups of mice which can exhibit 3 different exclusive behaviors. What test could I use to compare all 4 groups at once (something similar to an ANOVA but to compare distributions)? So...

09 April 2018 8,732 2 View

How well can an established immune synapse withstand mechanical external forces?

Hi all, Recently I have been working on analyzing the immune synapse formation using imaging flow cytometry. I co-culture mouse APCs and T cells and subsequently perform fixation/permeabilization...

04 April 2018 7,869 2 View

How can we remove the slime capsule from microalgae?

We are trying to get DNA out of microalgae, but we can't get them to lyse. We suspect this is because the slime capsule is preventing cellulase from reaching the cell wall. Does anyone have any...

04 October 2016 1,688 3 View

How to stop protein precipitation from pH change in Hi5 cell culture?

We have been using the Baculovirus system for protein expression. When we purify protein from Hi5s, we have to change the pH from about 6.3 or less to 8.0 for purification. When we change the pH...

24 May 2016 1,639 5 View

How do gabor filters detect edge?

I have read materials on gabor filters recently and get confused about this. A Gabor filter g(x,y,sigma,theta,omega) is the product of a gaussian window and a sinusoid function. When apply this...

26 April 2016 1,328 10 View

How to adjust a covariate in meta-analysis?

Suppose I am meta-analyzing proportions using their logit transformations. We did a meta-regression of the covariates age, gender, and disease severity score in studies and get respective beta...

11 January 2021 9,409 4 View

Writing participant characteristics in method while being LGBTQI+ sensitive?

I apologize in advance if I come across as ignorant. I am trying my best to educate myself on LGBTQI+ community and how to write them in academic writing. I have a sample of X participants in a...

29 December 2020 9,011 3 View

Hi! What is the best way to define/specify content-related factors and style-related factors in terms of language variation / gender differences?

28 December 2020 4,409 3 View

Which type of analysis should I run on SPSS? + confusing error message?

I have been advised to run a MANOVA for the data I have collected for my thesis. In trying to do this, I have encountered some issues. I am analyzing 4 IVs: Personality trait X, split into two...

12 December 2020 5,506 3 View

What is the best graph to compare mean before and after an event?

Hi, I'm doing Data Analysis coursework regarding diet and i want to compare the mean for each gender before and after said diet. However, I'm not sure how to present the data in the most...

03 December 2020 840 5 View

How can one integrate gender into designing any architectural space? are there any examples of it in the architectural practice?

I am doing my undergrad thesis on gender integration in architecture and could use a few views from people with different perspectives.

03 December 2020 8,378 11 View

Is it possible to receive cited research papers on "Discourse Analysis of Identity Reflection in Mass Media?

Research in "Discourse Analysis of Identity Reflection in Mass Media" will be quite beneficial for me. Any Identity of Any Nation/Nationality.

26 November 2020 475 3 View

Would you please help me find some scholar articles about Gender in teaching and learning?

I'm doing an assignment about Gender in teaching so I want to get some more information about Gender in teaching and learning.

19 November 2020 9,572 3 View

Can I use a decade old event as case study in order to support a hypothesis for a research?

I am working on extreme climate events and gender inequality by critically analyzing existing studies followed by a case study to support the hypothesis. The case study is based on the year 2000....

15 November 2020 5,244 6 View

How do we interpret the following gender differentiated data?

Hi, We are analyzing data and have found the following finding. The results show that men and women hold equal responsibilities for many aspects of forest management and decision making in the...

27 October 2020 6,399 4 View