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What is the prepared reference material that can be used in inductively coupled plasma optical emission spectrometry (ICP-OES) to measure REE and U ?

What is the prepared reference material that can be used in the ICPE-9820 Shimadzu Japan instrument, which employs inductively coupled plasma optical emission spectrometry (ICP-OES) to measure...

06 August 2024 1,896 1 View

How to produce homogeneous reference gene bands in Western Blot?

Hello, I have been performing Western Blots to study the protein levels of a rodent skin and muscle sample. However, I have not been able to produce homogenous reference bands. I suspect that...

26 June 2024 5,913 3 View

Cancer biology reference book?

Anyone can suggest me a good reference book on cancer biology that covers drug delivery and discovery as well as basic concenpts of cancer biology?

25 June 2024 9,245 1 View

Is it advisable to run reference genes separately from target genes in a comparative Ct analysis on ABI StepOnePlus due to logistical constraints?

Hi Everyone, I'm reaching out for some guidance regarding conducting a comparative Ct analysis with the ABI StepOnePlus system, and I hope you can help shed some light on my dilemma. Here's the...

11 June 2024 4,044 3 View

Why am I having huge shifts in reference genes Cq's?

I was recently performing a great deal of qPCRs on adipose tissue derived RNA. I had experimental samples from sick, and control samples from healthy individuals. I used the same chemistry for all...

04 June 2024 6,924 0 View

Could someone help a right procedure for TGA analysis of cement?

I analyzed cement samples exposed and unexposed to moisture over various periods. The TGA results of these samples showed variation with each repetition. However, I consistently used calcium...

22 May 2024 1,339 3 View

What to do with primer efficiencies ?

Hello - planning a SYBR Green qPCR study. I have primer efficiencies, including for reference genes, from 90-105% I am planning to use efficiency correction in my calculations - do I correct for...

15 May 2024 3,370 0 View

Need help for cq values in Rt-qpcr?

cq values for my reference coming like 22 and cq value of my target gene coming as 23 and 24 around? can i considered this value for future calculation? or what else i can do to get better cq...

14 May 2024 6,081 6 View

Window size TSAD (Time Series Anomaly Detection) ?

Dear all, One of the key points in TSAD (Time Series Anomaly Detection) when using a sliding window is the size of the window. For (quasi)-periodic time series, methods such as AutoPeriod,...

13 May 2024 920 3 View

Problem for methylation detection using qPCR?

Despite satisfactory qPCR curves for the positive control and calibrator, I observe a lack of signal from the VIC dye (reference gene) in my experimental samples after bisulfite conversion. I have...

13 May 2024 8,026 2 View