F6PPK Test, what can we use instead fructose 6 phosphate

Shamsi Saad Shamsi @Shamsi-Saad-Shamsi

01 January 1970 0 7K Report

as F6P in pretty expensive, what can we use instead in F6PPK as substrate

many thanks

Badges
Science topic

Similar topics
Geoscience
Asia
India

More Shamsi Saad Shamsi's questions See All

How to avoid polypyrrole deposit on counter electrode?

Hi all, Currently, I electrodeposited polypyrrole on carbon electrode but I got some problem when polypyrrole also deposited on the surface of the counter electrode. The concentration of pyrrole...

05 February 2021 2,103 3 View

How to Extract DNA from dried rhizomes, wood, galls etc. stored for a long time?

Dear Respectable Scientists, I have been trying to extract DNA from above mentioned sources using different CTAB/SDS Buffer Compositions and purifying agents but I am not able to extract DNA from...

12 January 2021 5,899 8 View

How to carry out Fisher's Exact Test in SPSS for multiple Response Dataset?

I have a dataset including Categorical variables and involves multiple response variable. 20% of the cells in cross tab has less than 5 frequencies and hence I cannot carry out a Chi-square Test....

26 December 2020 4,180 3 View

CLOSED Behavioral changes due to Covid-19?

Hello fellow researchers around the globe :) Happy Holidays!! We are conducting a survey on understanding how device usage during everyday life has developed amid the Covid-19...

21 December 2020 5,363 4 View

Does the Chinese navigation satellite system (Beidou) become global or still regional?

I know that this system was regional before, thus covering specific areas not alla the global

09 December 2020 3,343 3 View

Do the exams for students in electronic education have the same credibility in terms of measuring and evaluating learning as traditional education?

Educators around the world face a new challenge of how to conduct exams to assess what students have learned after the shift towards e-learning following the closure of nearly all universities...

21 September 2020 4,180 15 View

What Features Should an Online Exam System Include?

ith the development of Internet technology, online examination has become more Polar because it helps people save a great deal of energy and time. As an effective and efficient method of teaching...

21 September 2020 2,814 12 View

How can heat transfer coefficient on shell side of Shell and tube heat exchange can be calculated using Ansys Fluent?

I have to calculate heat transfer co-efficient on shell side and plot its variation along the length of heat exchanger

07 September 2020 9,078 6 View

How to get rid of negative Pressure? and my pressure readings are not accurate what can be the cause?

I am simulating shell and tube heat ex-changer i have mass flow inlet and pressure oulet (zero gauge) but my pressure readings are not accurate i have tried velocity inlet but results are same....

07 September 2020 7,298 11 View

AST result in spss ?

hello dear microbiologist Am kinda new and still learning SPSS I want to know what kind of tests should I run for AST results I see lots of paper use chi square. And can anyone provide me with...

10 August 2020 9,911 4 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why daily dexamethasone given to rats fed high fat/fructose/salt cause very high mortality rate?

rats were given daily dexamethasone in drinking water corresponding to 0.5 mg/kg or 2 mg/kg. These rats are simultaneously fed 25% fat, 3% salt in diet and 10% fructose in drinking water but...

15 February 2021 7,634 2 View

Phosphate buffer with different pH conditions: HCl or NaH2PO4?

Hi all, I just encountered this dogma; when adjusting pH of phosphate buffers, I've been using HCl/NaOH to in/decrease pH of buffers and recently I was told that HCl/NaOH can't be used to adjust...

08 February 2021 9,070 5 View

Why FTIR can not detect bases of nucleic acid in extracellular polymeric substances (EPS)?

I characterized functional groups of EPS using FTIR. Goups of sugars and proteins (e.g., -OH, amide, -COOH) as well as phosphate group of nucleic acid were readily detected. However, peaks of...

05 February 2021 4,405 1 View

Why sometimes current- voltage plot in chemiresistors/ FET does not pass through the origin?

I am trying to measure resistance from I-V curve from a chemiresistor/ FET sensor with a keithly sourcemeter. The substrate is soaked/ wet with protein and phosphate buffer only. But the I-V line...

24 January 2021 8,009 3 View

How to make a 100mM potassium phosphate buffer at pH 2.5?

Do I just need to add my basic and acidic potassium phosphates to get the lowest pH achieved by mixing those (I think it is around pH 5.8), and then lower the pH further through addition of HCl?...

06 January 2021 7,231 2 View

Can total phosphate (TP) in soil be determined with UV-Vis Spectrophotometer?

Dear All, I would like to know if total phosphate (TP) and total nitrogen (TN) in soil can be determined with UV-Vis spectrophotometer or not. If somebody knows the method, I would like to get...

03 January 2021 7,662 6 View

During my work in the laboratory?

During my work in the laboratory, I noticed that fructose, starch and dextrin did not give positive results with their statements such as iodine and sulwanof when prepared 3 to 5 days ago. Why?

25 December 2020 8,390 5 View

In ELISA should we use p-nitrophenyl phosphate (pNPP) substrate mixed in distilled water or we should mix it in Diethanolamine buffer?

We are performing an ELISA experiment to determine the binding of Complement antibodies against P. aeruginosa. We have pNPP substrate (Thermo Scientific cat # 34045) instead of pNPP tablets. We...

22 December 2020 2,020 3 View

Can xylose be formed from fructose over acid catalysts?

I am new in the field of sugar chemistry. I wanted to know if it is possible for fructose to be converted to xylose over acidic conditions. I saw in some schemes a pathway from fructose to xylose...

07 December 2020 5,247 3 View