Extraction of compound from tissuees by trypsin?

02 March 2020 0 6K Report

Dear colleagues

Is it possible to extract different compounds (surely not proteins! :) from tissues such as heart brain etc.... for example digest tissues and extract a drug?

Have anyone experience about this?

Best Regards

Badges
Science topic

Similar topics
Mathematical Sciences
Graphs

More Reza Heidari's questions See All

If the emission of all greenhouse gases is stopped now, will the temperature of the earth continue to rise?

Here's a serious question: If we were to stop emitting all greenhouse gases right now, would the Earth's temperature start to cool down, or would the existing greenhouse gases continue to warm the...

30 July 2024 5,123 4 View

How to prepare 100ml solution of 2.5% Glutraldehyde in 0.1M PBS(phosphate buffer) pH about 7, from 25% Glutradehyde and 1X PBS?

final solution volume is 100ml

03 June 2024 417 0 View

How can i use hexamethyldisilazane(HMDS) for drying instead of Co2 and freez drying for SEM analysis of rice anther and pollen?

could you please help with a protocol? should i do it directly on the aluminium stub after dehydration with 100% alcohol or not? should i release the pollen or whole anther is better and dehisce...

03 June 2024 4,793 8 View

What is the best method to enhance the surface hardness of cutting tools made from SPK steel?

SPK tool steels are used for cutting wires made from low carbon steels. We are searching for a cheap and effective method for increasing the cutting characteristics of the cutters.

14 May 2024 4,948 0 View

Constant Reciprocal of Step Size?

Hello I recently encountered a problem with the reciprocal of step size plot in Comsol Multiphysics. After a certain time (seconds) in a time-dependent solver, the plot oscillations will be...

04 May 2024 7,278 0 View

Poor viability of T cell after transduction with CAR Lentivirus!?

Hi everyone I produced a batch of virus , enriched it with centrifugation, and titrated it with T cells after 48h. For my experiments I want to use MOI 3 and that's equal to 1/12 dilution. but at...

02 May 2024 9,727 3 View

Are you interested in submitting papers to a new journal called "InfoScience Trends"?

InfoScience Trends offers comprehensive scientific content that delves into various facets of information science research. This includes but is not limited to topics such as information...

26 April 2024 9,521 1 View

Does the color of pan traps affect the sampling of flies from the superfamily Bombylioidea?

Garret, 2020 used traps of blue, yellow, and white colors for sampling and collected several specimens.

06 April 2024 6,995 2 View

Laboratory procedures for identifying the concentrations of formic acid and acetic acid in aqueous solutions???

What are the laboratory procedures for identifying the concentrations of formic acid and acetic acid in aqueous solutions using titration, spectrophotometry, or gas chromatography methods...

06 April 2024 187 3 View

Is it possible to produce a very small amount of volatile oil along with condensed gas? If so, could you please provide a real example?

Is it possible to produce a very small amount of volatile oil along with condensed gas? If so, could you please provide a real example? Thank you.

04 April 2024 1,411 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Do You Know Untreated TMJ Affects Your Brain And Entire Body?

04 August 2024 6,282 1 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

Recovery rate after peptide desalting using Sep-pack C18 1 cc Vac Cartridge?

Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?

31 July 2024 6,544 11 View

Can telekinesis, telepathy, and prediction be achieved through science?

Information in The Three Dimensions of Time.Information in The Three Dimensions of Time Information Transfer based on Brains Entanglement.Information Transfer based on Brains Entanglement Time...

28 July 2024 7,522 0 View

Why only alpha wave of brain are found in some patients?

I have found an EEG where only alpha waves are present. Beta waves are not found in active patients. What interpretations ?

26 July 2024 4,741 1 View