Hi Baki,

Yes, you will loose exosomes each time. The less you freeze your sample, the better!

Best,

Julia

Freezing and thawing process do damage exosomes. After the extraction is better to keep them at -80ºC for long periods of time (above months) at -20 for a few weeks and at 4ºC if you're planning on using them during the week.

Here is a paper I recommend for that question

Article Effect of pH, temperature and freezing-thawing on quantity c...

Thank you Carla !...

exosomes are pretty durable entities, so single freeze/thaw cycle does not hurt them much. And also in some cases we receive frozen plasma or serum samples- for which we cant control harvesting/storage/transportation (ideally of course you want to process everything fresh or stored at 4C for 1-2 days)

Thank you Alexander. I have the same thoughts but wanted to be sure.