I have used the gold nanoparticles (AuNPs) for biomecal applications

Where are you from?

Can you send your paper to me on my email

Plz read opprtunities and threats from nanotechnology in health, food, agriculture and environment by Ricardo Molins

My work is mainly concentrated on silver nanoparticles and their effects on seeds. work is in progress

Silver or gold, since now there has been no negative effect discovered on germination. Germination takes place from inside to out (Seed) so this is not surprising. I strongly advise to test nano particles on protoplast culture (Plant regenerative cell culture). Protoplast cells are vulnerable to nanoparticles so effects could be observed. If I have chance I will test in the future myself.

Try selenium nanoparticles produced by lactic acid bacteria. "Process for producing elemental selenium nanospheres". Patent 8003071 Issued on August 23, 2011 by Prokisch J. and Zommara M.

Can i get a rigid answer to my question?

Iam form iran

According to my experience (solid ones only) silver, zinc, iron and gold (Rarely gold but not found significant) is used on soya, rice and Arabidopsis thaliana and no adverse effect is found.

Has zinc been tried with protoplast culture

Not widely. The exposure can only be applied by a special method otherwise nanoparticle penetration can not take place in plant cells.

You can find some nanoparticles that show toxicity in seed germination and root growth. 1.Phytotoxicity of nanoparticles: Inhibition of seed germination and root growth. Environmental Pollution 150 (2007) 243 - 250

2. Effect of Nano-Silver on Cell Division and Mitotic Chromosomes: A Prefatory Siren . K. Babu, M. Deepa, S. G. Shankar & S. Rai :. The Internet Journal of Nanotechnology. 2008 Volume 2 Number 2

Application of nano science is pratcticable in seed coating to minimize the chemical usage; it improves the image of seed and enhance quality; scientists are working in this regard, results are awaiting ...!