Sac1 phosphatase (Sac1p) exists in ER and golgi. It undergoes this reaction : PI(4)P -> PIP. Certain authors said that Sac1p acty in cis in ER and others said that Sac1p is in ER but act in trans on golgi.
Sac1 localizes to both endoplasmic reticulum and cis-Golgi in proliferating mammalian cells; it is absent from the trans-Golgi network.
For my research, I must use AKTA protein purifier and UNICORN software but I don't understand them. How do you use them? Can you help me ? Do you have a method or a protocol ? Thanks in advance....
02 March 2014 2,298 4 View
In the paper "Mutation of the His ligand in mitoNEET stabilizes the 2Fe-2S cluster despite conformational heterogeneity in the ligand environment" by Conlan Andrea R (2011) published in Acta...
01 February 2014 7,869 1 View
C41(DE3) and C43(DE3) strains derived of BL21(DE3) strain E.coli. I am searching for where the mutated genes are in C41 and C43 strains and also the function of these genes in the wild type BL21...
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I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
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In My article: The Extended Pedersen hypothesis (published, February 1988 Clinical Physiology and Biochemistry 6(2):68-73,) there is mistake of the name of first Author: it is not Catherine...
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I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...
07 July 2024 8,275 5 View
I have extracted essential oil from a medicinal plant and want to make several concentrations for some biological activities (Antidiabetic assay). How can i prepare the several concentrations to...
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Unluckily, just similar to an expensive car, your brain can be damaged if you ingest anything other than premium fuel.
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It is known that patients with desminopathy often die from pneumonia. Have pathomorphological studies of the lungs been performed in patients with desminopathy?
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Hi! I'm doing a renilla luciferase assay with coelenterazine and HEK293T cells transfected with AP-1 renilla luciferase. I'm wondering if the cells need to be lysed prior to adding coelenterazine...
13 June 2024 9,936 5 View
Hi! I have performed several immunofluorescences of cultured ARPE-19 cells with different antibodies expecting in each one a cytoplasmic localization. However, it seems to localize in the...
06 June 2024 7,764 1 View
I have purchased Acetylcholiesterase from Electrophus electricus (electric eel), C3389-2KU, following details are written on the enzyme vial. Type-VI S lyophilized powder 200-1000 Units/mg...
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What is the difference between absorption and adsorption?
30 May 2024 4,447 27 View