@ Bander, the answer is no, rather liquid nitrogen storage preserves DNA quality over the course of decades, whereas storage at -20 °C and -80 °C can prevent degradation for months or years. I think the most effective DNA extraction method is Phenol-chloroform method. The yield and quality of DNA obtained by the PCI method are very good if we perform it well. The method is also referred to as a phenol-chloroform and isoamyl alcohol or PCI method of DNA extraction.
Thank you Professor Tarafdar for answering and your Helpful information 🙂.
Liquid nitrogen is one of the cryogenic liquids commonly used in research labs, I,e preserving of DNA etc. There are so many conditions for preserve DNA. Fast freezing technique induces a low percent of DNA degradation comparing to slow freezing technique. The crystals ice performing mechanical action that could cause the membranes rupture and DNA degradation.
Depending upon the type of sample, every DNA extraction method varies, DNA extraction methods are broadly categorized into two categories:
1. Chemical-based DNA extraction method.
2. Solid-phase DNA extraction method.
The chemical DNA extraction methods are also known as solution-based methods whilst solid-phase DNA extraction is a type of physical method
Several different types of DNA extraction methods are Phenol-chloroform isoamyl alcohol, Proteinase K, CTAB method, spin column-based methods and magnetic bead-based technique. However, which method to use depends on the sample type and Purity & yield of DNA we want to obtain.
Phenol-chloroform method of DNA extraction .
Thank you Professor. Prem Baboo for answering the question & that was really helpful🙂.
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