How do you get around this AutoDock Vina error?

I get the following error messages after running the simulation: Sorry, there are no Gasteiger parameters available for atom ligandx.ligand:A:lig1:O7 AutoDock Vina ligand docking initiated for...

26 January 2021 3,822 3 View

Speaking of the Brazilian test of Lobo Carneiro, which other researchers did similar tests?

22 October 2020 4,536 2 View

What is the best etchant for 3d-transitional metals high-entropy alloys?

I would like to reveal the as-cast dendrite microstucture.

30 January 2019 9,690 3 View

Metagenome Contig Taxonomic Classification Approaches?

If you were a researcher interested in investigating a particular group of prokaryotes (e.g. Cyanobacteria) from an environmental sequencing project, which of the following approaches to taxonomy...

26 October 2018 7,027 3 View

Is there an easy way to determine the area of a raster inside a polygon/shapefile?

I have a (1) polygon/shapefile of administrative boundaries and a (2) a raster with different categories (e.g. 1, 2, 3, 4, 5,) I would like to determine the area of the raster (only category 1)...

18 October 2018 4,545 5 View

Does the concentration of purified pcr product affect the result of NGS community distribution?

Although the loading mass for NGS would be the same, I am just thinking if it matters if my DNA concentrations after purification varies a lot (e.g. 20 vs 200 ng/uL)?

21 January 2017 1,798 1 View

What spider species is this?

Have a prosperous new year to everyone! What spider species is this found in a commercial swimming resort in Jasaan, Misamis Oriental, Philippines. Please help in identification

01 January 2017 5,295 10 View

What are current teaching strategies to teach various topics in Chemistry?

Can you share some teaching methodologies/strategies to teach specific topics in general chemistry?

30 November 2016 507 6 View

Diesel Combustion - Effect of pressure on boiling point temperature?

14 August 2016 2,806 5 View

How can one maximize both atomic absorption spectroscopy (AAS), and x-ray fluorescence (XRF) as techniques in determining and visualizing Pb content?

07 July 2016 8,766 4 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

Why can I not detect other targets than GAPDH?

Hello, we're trying to detect Keratin 14 and 15 in Keratinocytes as well as FN-1 and CD90/THY1 in Fibroblasts from human skin. As reference gene GAPDH and Vinculin are used. In our first...

07 January 2021 6,652 3 View

Besides, DMSO, EDTA, and K2Cr2O7 to scavenge the OH radicals, holes, and electrons respectively what are the other chemicals being used for scavengin?

Besides, DMSO, EDTA, and K2Cr2O7 to scavenge the OH radicals, holes, and electrons respectively what are the other chemicals being used for scavenging? Please share the files with complete...

30 November 2020 4,935 1 View

How can I synthesis heparin nano particle?

I want to synthesize iron nanoparticles with heparin, but I have trouble synthesizing First of all, do I have to use a freezer drying for drying of product and it is not impossible use from...

16 November 2020 9,455 2 View

Can you help me figure out how many EDTA I need to add in the solution?

Composition 2X Loading dye for RNA electrophoresis 95% formamide 0.025% SDS 0.025% bromophenol blue 0.025% xylene cyanol FF 0.025% ethidium bromide 0.5 mM EDTA. I wanna to prepare 10 ml of the...

10 November 2020 4,759 3 View

Does PFA transcardial perfusion artefactually stretch the rodent heart?

Hi everyone I'm comparing aorta/ventricle wall thickness of mouse. Perfusion was done to minimise auto fluorescence during confocal IF. Question is whether transcardial perfusion artefactually...

28 October 2020 8,059 2 View

Causes of poor cell viability of WBCs after RBC lysis with 1X FACs Lyse buffer?

We are performing a whole blood lysis procedure on heparinized blood (3mL) to lyse RBCs and later remain with WBCs that we count and freeze down and later cryopreserved in liquid nitrogen for...

20 October 2020 7,677 6 View

Could bone tissue be saved in 70% EtOH after decalcification before processing?

I got the mouse tibia and femur tissue harvested, fixed with formalin 48h and decalcified with 14% EDTA 72h before paraffin embedding. This protocol works well for both HE and IHC. Now, for the...

18 October 2020 5,318 1 View

Does anyone experienced EDTA salts precipitation in DMSO?

I added 2 mL of 0.1 M EDTA aqueous solution pH 6, to a solution of 3 mL pure DMSO and instantaneously I observed salt precipitation in solution. Is this happening because the pH of the EDTA...

17 October 2020 6,329 1 View

How do you create a 0.5M EDTA disodium salt solution at pH=7.4?

I have been trying to make a 0.5M EDTA solution by dissolving EDTA disodium salt dihydrate in 500mL of purified water and adjusting the pH to ~8.0 so that the salt dissolves. To aid in the...

12 October 2020 3,242 2 View