In my recent project study, I am getting absorbance value for distilled water > 0 (mostly around 0.05). Distilled water free from all sort of ions or entities should show zero absorbance according to me.
Can anyone explain the phenomenon?
I think you should try deionized water instead of distilled water.
The distilled water deionized by the effect of uv lamp and produce distilled water grade A.
Your distillation process can be not very good, you cuvette can be not enough clean.
DI water has very small absorbance value (1 x E5) which is almost close to zero, however it depends on the wavelength. When the wavelength is 515nm, the absorbance has its maximum value.
Distilled water is free of ions. Also, when a beam of light travels through a mixture of waves in a material solution, the light falling on the material may be distributed in three directions: absorbent light, dim light and opposite light.
I think you should try deionized water instead of distilled water.
The distilled water deionized by the effect of uv lamp and produce distilled water grade A.
Water absorbs faintly around 800 nm.
https://en.wikipedia.org/wiki/File:Absorption_spectrum_of_liquid_water.png
In most cases a non zero signal from solvent is the result of inaccurate baseline correction.
Hello Tuhin, I am attaching a file, just check 412 page onwards.
I considered absorbance of chloroform as the blank and found that absorbance of Distilled water is non-zero. From my seniors I got confirmed that chloroform absorbs light. But my question is that distilled waterr fre from all minerals & impurities should show zero absorbance.
Just for example, in one set of experriments without blanking at first, I got absorbance value 1) CHloroform: 0.049
2) Distilled water : 0.051
My question is with the second value.
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