How to perform electrophoresis and western blotting for high molecular weight proteins such as Ryr2?

17 August 2017 6,103 7 View

Low intensity of bands in DGGE

Hi everybody! I don't know why gel bands appear with low intensity. I use 50 uL SYBR Green I diluted in 200 mL TAE 1X to stain the gel. Also, gel is staining during 1 hour in agitation and...

11 July 2017 2,679 4 View

ImageJ to analyze DGGE gel???

Hello everybody! I'm working with PCR-DGGE to study gut microbiota composition, and I want to know if it's possible to analyze DGGE gel with ImageJ. Some suggestions about softwares that you use...

09 March 2017 3,264 3 View

Design primers for PCR-DGGE???

07 March 2017 1,301 3 View

How do I create amide bonds between graphene oxide and chitosan?

I want to form amide linkages between the carboxylic acid groups of graphene oxide (GO) and the amine groups of chitosan (CS) in order to create a CS/GO composite. What is the best catalyst and...

01 September 2016 629 1 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Any suggestions for a website that allows you to create questionnaires and gather unlimited data (Totally free)?

I have found only four of them; www.surveyplanet.com www.online-anket.gen.tr www.surveey.com and of course google forms. Expecting to see your suggestions if there is any. Thank you

28 February 2021 3,237 3 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Do I need to ask permission to use pictures for my questionnaire?

I would like to use some photo from a government website for my questionnaire. But, different photo is from the different owner. So, should I just get permission from that government body or still...

23 February 2021 1,631 3 View

How can I get the coding gene sequences of Hela and U2OS cell lines? Is mRNA sequence of Hsp90 alpha tissue specific??

I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...

22 February 2021 1,853 3 View

How do you interpret multiple meta-regression in STATA?

Please could someone recommend a resource that explains performing meta-regression with multiple moderators. I have read the STATA META ANALYSIS REFERENCE MANUAL, but this only gives examples of...

17 February 2021 7,376 1 View

Can anyone pls provide me information regarding the libraries where from i can get the absorption data of different materials used in solar cells??

I want to create .abs file ( absorption) for simulating solar cell in SCAPS. Can anyone pls provide me information regarding the libraries (websites) where from i can get the absorption data of...

10 February 2021 6,034 7 View

Glucose 6 phosphate dehydrogenase deficiency & NSAIDs - is this combination harmless ?

G6PD deficiency is quite common in our anesthesia practice in the middle east. Intraop/postop analgesia in them is an issue here. Many websites/books give contrasting advise with former advocating...

10 February 2021 4,817 3 View