Why do I keep getting smudged and wavy bands in certain lanes of my western blots?

Hi everyone, Been doing western blots for years now, recently however, whenever I run 2 gels, one of the gels would have a few lanes that exhibit wavy or smudgy bands at around 50 kDa (see...

16 November 2020 3,759 11 View

How to use Clamping and interpret its outcomes in MAXENT?

For my Masters project I have been creating SDMs using MAXENT, and the next stage involves using future climate projections in the 'projection layers directory' to see potential future...

25 June 2020 2,658 6 View

Data and modeling for vapor-liquid metastable states, spinodals?

What publications are available with actual measured data on vapor-liquid metastable states and spinodals and the modeling thereof?

16 February 2020 9,000 1 View

I seem to have Protein stuck on my C8 column. How do I clean it properly?

Hi everyone, I've been trying to detect a Peptide Hormone (ca. 9 kDa) by RP-HPLC using H2O + 0,1% TFA and MeOH + 0,1% TFA as mobile phases with a flow rate of 1 mL/min, a C8 column with a...

07 April 2019 3,053 11 View

In Black hole theory, have mathematicians finally lost all connection with physics?

18 November 2018 4,730 38 View

Model Implied Instrumental Variables experiences?

MIIV (http://fgme2017.de/kenneth-bollen/) looks like a very promising approach. Are there any experiences from research practice? Application-oriented publications? When is it appropriate to use...

09 February 2018 174 1 View

Which is the best culture media for culturing macrophages?

08 October 2014 3,945 19 View

Is anyone familiar with In-vitro RNA transcription for long >6 kb transcripts?

20 September 2014 2,928 2 View

What kind of statistical test should I choose if I want to test regression to the mean on related data set?

I conducted an experiment to investigate the effect of a physical therapy treatment on the signal intensities of the 5 lumbar discs (L1-2 to L5-S1) on MR images. I found that the signal intensity...

07 March 2014 4,762 6 View

Has the speed of light been accurately measured in space, and would it be the same as on earth?

I believe there is reason to expect that the measure of the speed of light on the International Space Station would be greater than it is measured at the surface of the earth. My expectation is...

10 November 2013 2,652 32 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How could I process data in RCT to include it in meta-analysis?

I'm in the process of doing a meta-analysis and have encountered some problems with the RCT data. One of my outcom is muscle strength. In one study, I have three different measurements of muscle...

25 February 2021 7,603 3 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

Satellite orbit estimation using Recurrent Neural Network - what should I use?

Hello, I am a master's student studying in Yonsei Univeristy, Korea. I am trying to estimate the state of satellite, using Neural Network. Below is a simple flow of my study. 1. Train (t0 ~ t1)...

23 February 2021 6,383 3 View

Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations?

Dear colleagues, Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations? The question is mainly aimed at adolescents (pupils,...

23 February 2021 7,796 4 View

Any Differences in COVID-19 vaccines?

Are there any differences (outside of the clinical trials that led to their EUA) observed in the rate of infection among those who have received the Pfizer vs Moderna vaccine since the mass...

22 February 2021 9,727 6 View

How can I get the coding gene sequences of Hela and U2OS cell lines? Is mRNA sequence of Hsp90 alpha tissue specific??

I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...

22 February 2021 1,853 3 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View