What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View

How can I open a SXV4 file (SuperCROSS) in SPSS, R or STATA?

Hello everyone, I received some data from Statistics South Africa in an SXV4 format (SuperCROSS) and would like to open it in SPSS (or a similar programme if need be). I have tried opening it...

05 January 2021 2,463 4 View

Flow cytometric analysis of NBDG-uptake and intracellular staining possible?

Hello everyone, I am measuring glucose uptake into my cells by using NBDG and flow cytometry. Normally this works perfectly fine, but now I got some problems. When I fixed and permeabilised my...

25 October 2020 3,416 5 View

How can I analyse the modal ethnicity of a household and compare this to the ethnicity of the household head (SPSS)?

Hello everyone, I am working with a household survey and have both a person and a household file. I am using the household file for the main part of my analysis but want to explore the household...

21 July 2020 7,716 2 View

Who is working with Alzheimer's brain data?

Hi everyone, I am looking for a typical Alzheimer's brain for teaching purposes. I need a .nii file or similar. Of course the file should be showing a very typical brain and must be...

25 November 2019 7,917 3 View

What could be the Reason of Keratinocytes are growing in 75Flasks but not in same treated 96 Wells?

We work with OKG4 gingival Keratinocytes between Passage 13-18 and cultivating them on Collagen 4 in 75cm Flasks and implement them with 2x10*4 cells on 96 Well with 0,6cm growth area and 5x10*5...

18 November 2019 7,322 2 View

Moderated mediation in PROCESS - Interaction not sig?

Hello, I'm computing moderated mediations using PROCESS for SPSS. The interaction effect for the moderation is not significant, however, the entire model for the moderated (!) mediation is...

16 December 2018 7,307 2 View

Did anybody experience crystal formation in BrdU ELISA (Roche)?

We use the BrdU ELISA Kit from Roche a lot & never had any problems with it. Somehow, now we observe crystal formation (dark violett to black crystals) in the last steps of the assay... we did...

26 March 2017 3,668 2 View

How do you connect individual short tracks in FIJI Image J Trackmate?

Hey there, has someone used FIJI Image J Tackmate before and could give me some advice on altering automated tracks? I'm trying to determine swimming speed of larvae. Trackmate detects the larvae...

28 February 2016 7,894 2 View

Does anyone have experience with gene expression assays working with FFPE material?

05 June 2015 1,204 1 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View

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25 February 2021 5,713 1 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View

Is there an open source free software for text mining and sentiment analysis?

I am looking for a free software for text mining and sentiment analysis for my research on customer review mining (it involves calculating polarity of attributes,opinion oriented information...

25 February 2021 1,920 6 View