I have tried doing a primary embryonic neurons in chamber slide experiment. The neurons will grow when the chamber slide is PDL coated. However, when I try to do immunocytochemistry, the processes wash away/ break. Does anyone do acid etching on chamber slides? If so, what chamber slide brand do you use to do this? What do you do if you do not use acid etching?
Hi Britanie,
I use mostly primary hippocampal neurons from embryos and for some exp. I used chambers slide to visualize live neurons. After live acquisition, I tried fixing and staining for ICC. However, I never had this problem.
I used these chambers: Ibidi µ-Slide 4 Well (https://ibidi.com/chambered-coverslips/37--slide-4-well.html) and Ibidi µ-Slide 8 Well high Glass Bottom (https://ibidi.com/chambered-coverslips/252--slide-8-well-high-glass-bottom.html). Once I ordered an already coated chamber (PLL) and they worked pretty fine.
Which protocol do you use for fixation and staining?
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