I wants to measure mRNA expression in biopsies from human jejunum/ileum, and my end point after treatment is absorption. I am planning to measure expression of SGLT, FABP2, calbindin, and Tight junctions proteins, but maybe you have better ideas?
Thanks
I am not following the experiment. You want an mRNA marker that are known to change when the intestinal epithelium are absorbing something or do you want to measure absorption in blood and see if that correlates with the selected mRNA candidates?
Hi Hanne. I would suggest to use galactose as it uses SGLT-1 similar to glucose. best Thomas
The patients (short bowel patients) are treated with an agent and biopsies are taken before and after treatment. The patients are also going through balance studies (measuring intake/output for four days), which will give us the answer if the absorption is increased. But it would be great if we could measure some changes in mRNA markers of absorption after treatment. We hope that the agent would change the expression of e.g. tight junctions (as i does in animal experiments). So we are just looking for some good markers for absorption, that could be changed after treatment.
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