Hey guys, I'm looking for an efficient protocol to transfect patient-derived tumor cells with a high rate of transient transfection?

As you know it's quite tricky to get primary cells transfected at a relatively high rate!

31 January 2021 3,580 4 View

What would be the best option to dock a number of peptides to a protein?

I have been using PyRx to dock peptides to a protein. Initially the numbers were small so it was okay. But now I have to dock over 500 peptides and doing so manually would take forever. My...

10 January 2021 3,330 4 View

What is the effect of drought and silicon stress on soil pH and EC?

What is the effect of drought and silicon stress on soil pH and EC?

15 November 2020 3,971 5 View

How do I get the number of water molecules in a carbon nanotube?

12 September 2020 886 6 View

How can I access the Seqscape software 2.7 ?

Hi, I need Seqscape software 2.7 to analyze DNA sequences because this version supports 3500 sequencers. Please help me how can I access this version of the software? Thanks

17 August 2020 4,693 3 View

Is it possible to use an accumulator as the main supply of oil pressure instead of the pump in a power steering system?

it means the oil pump to be sometimes able to turn off during the driving cycle, and if it is possible, how efficient would it be? (more particularly in heavy vehicles like a bus).

03 April 2020 6,765 1 View

Does anyone have an Excel file on how to computer questionnaire validity by Lawshe?

When we want to measure the validity of a questionnaire, we usually measure by Lawshe formula- CVR & CVI. I want to know that if anyone has an Excel computing file for measuring the validity...

02 March 2020 6,068 1 View

MiRNeasy Serum/Plasma kit abnormally low OD?

We have purified RNA samples using miRNeasy Serum/ Plasma kit using RNase free solutions but still, we have got low OD of purified samples ( 98 and 56 microgram/mL). which actions should be measured?

21 September 2017 1,067 2 View

The method was applied by gaussian software for calculations of BSSE

24 June 2017 7,758 3 View

What reactions is used in MTP(methanol to propylene) process?

All of the reactions in this process

15 May 2017 8,722 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

No title

02 March 2021 3,060 3 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

No title

01 March 2021 3,905 5 View

How to Balance Bench work with Reading and Writing?

I am a second-year grad student. I am finding it difficult finding time to read literature and write (things like qualifying exam, thesis, grants). I find myself so busy with bench work that I...

01 March 2021 3,325 4 View