Does anybody know some substrates that can hydrolyze and produce chromogenic products?

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Snehal Ingale

p-nitrophenol-B-D-Glucuronide is such substrate which on hydrolysis releases nitrophenol which is coloured.

Adam B Shapiro

What enzyme do you want to test for?

Ali Ghanbari

I want use this substrate on to microbial culture media.

Understood, but is there a specific enzyme activity you want to test for in the media? For example, X-gal tests for the presence of beta-galactosidase.