Does anybody know how to do single channel conductance analysis using Axograph?

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Ezequiel Marron Fernandez de Velasco

Hi Refik,

I am not sure if you can use Axograph to do singles analysis but you can definitively do it using Clampex.

Ezequiel

Refik Kanjhan

Thanks Ezequiel,

You probably right. I have not done it before, so I though I ask, just in case there is a way around it. Thanks for confirming my thoughts.

cheers, Refik

Simon Kalita-de Croft

The parameters you need to calculate the conductance should be straightforward to obtain using axograph?

Which parameters specifically are you looking to measure?

Simon

Thanks Simon,

I want to analyze single channel conductane of single hemichannels. There is no conductanca analysis function in Axograph. Have you done single channels conductance analysis before? If yes, perhaps we can meet for a coffee and you can tell me about it.

cheers,

Refik

Hi Refik

I have not specifically done single channel conductance, but I do use axograph regularly so I might be able to help.

In which lab do you work at QBI?

Hi Simon,

I am at the School of Biomedical Sciences, MacGregor Building. I am actually planning to transfer my data to Clampfit which seem to have conductance analysis within the software. If you are around I'd like to meet, since there are not that many electrophsyiologists around these days. Are you from NZ?

Thanks,

refik

Yes I"m from NZ, but working at QBI now.

Sure I'd love to meet a fellow electrophysiologist!

I'm around on Friday afternoon if that suits you?

SImon

Here is the e-mail answer I got from John Clements who wrote Axograph software.

There is no dedicated single-channel analysis built into AxoGraph.

However, you could use the event detection features to estimate these parameters, assuming the recordings have a reasonable signal-to-noise ratio. You could set up to detect channel opening, and capture the open events into a new window. You could then measure the amplitudes and open times for the captured events. Also, if you average the captured events, you’ll get a decaying current with peak amplitude approximately equal to the channel current, and decay related to the mean open time. I realise this is not as refined or direct as a dedicated single-channel analysis program, but it would definitely give you some useful numbers from your data.