My dsDNA sample might be degraded and might contain ssDNA as well. How can I easily check (and quantify) if my DNA is stil double stranded?

I have checked a sample of salmon DNA (provided as 'solid' dsDNA and brought into TE-buffer up to a certain concentration) with the Nanodrop. According to the measurment (performed several times),...

04 April 2016 9,236 4 View

Additional staining of the gel after real-time PCR?

When I perform a real-time PCR experiment (with for instance SYBR Green or EvaGreen), would you also stain the gel (to check the amplicon lenght) with this dye or is the presence of the dye from...

22 March 2016 1,239 8 View

Does anybody know anything about healing of injuries in compound eyes (all comments, pictures, references are welcome ...)?

19 July 2015 2,355 6 View

Why is my SH-SY5Y not gaining a differentiated (neuronal) phenotype after treatment with 12-O-tetradecanoyl-phorbol-13 acetate (TPA or MPA).

I am treating 1-week cultured SH-Sy5Y with 80 nM TPA for one week, replacing the media with the TPA twice a week according to (Presgraves, 2004). Instead of growing neurites, I notice that the...

18 June 2013 2,657 6 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View

No title

22 February 2021 9,932 11 View