sorry it was to cut the leaves to observe chloroplasts..etc under fluorescence microscope with an aquatic plant that has thick leaves

Thanks indeed a razor was used to cut the leaves but the results are not fantastic as still very thick

 Dear Brigitte,

I would ask silvia.mazzuca who is an expert in Marine Plants and extracts chloroplasts from theses species.

Norbert

Dear Norbert

Thanks a lot for this information.

Best wishes

Brigitte

Dear Brigitte

I hope the reference below can help you.

http://www.sciencedirect.com/science/article/pii/S0168945204003401

we want to observe autofluorescence of chloroplasts and to use some specfic fluoresencent dyes as well to merge pictures

maybe someone in the group of Hendrik Schubert (University of Rostock) can help you? he has done a lot of charaphyte research.

Thanks for this advice. 

Assuming the leaves are soft you can cut nice sections with a vibratome. You should be able to use either fresh or fixed tissue for this.