Doable bioassay for neurotoxicology/neuroprotective properies of natural produts

More Ourlad Alzeus Gaddi Tantengco's questions See All

Will DAPI filter detect cyan fluorescent protein?

I am using a fluorescence microscope with DAPI filter, here are the specifications: Excitation wavelength: 360/40 nm Emission wavelength: 460/50 nm Dichroic mirror wavelength: 400 nm I want to...

04 June 2020 5,841 2 View

Do you know researchers on health communication particularly in the use of social media for health promotion and education?

Hello. Do yu know anyone working on health communication field? I have been using Facebook page to share health information. I already have 300,000 Facebook page followers. I recently started...

23 May 2019 2,468 5 View

How to release viable content from extracellular vesicles?

I would like to know if you have idea how to lyse the membrane of the extracellular vesicle without damaging its contents? I am looking at bacteria transported via EVs and I want to isolate these...

14 April 2019 2,997 2 View

Where can I get T Maze for Drosophila behavioral assays?

Do you know where can I get Drosophila T Maze for memory and learning assay? I really need it for my research. Or do you know how can I create a makeshift T Maze? Thank you very much

13 September 2016 1,584 3 View

Can I combine prevalence odds ratio from cross sectional studies and odds ratio from case-control studies in a meta-analysis?

I want to know if it is acceptable to combine cross-sectional studies (with the prevalence from the case group and control group) and case-control studies in a meta-analysis. I have seen several...

01 January 1970 3,512 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Necessary tube lenght to cool Natural gas in a dry-ice cooling bath?

I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...

27 February 2021 8,128 1 View

Exit velocity of natural gas from pressurized tank?

Hi Guys. I'm a mechanical engineer and have a bit trouble, would be very glad if you could help me with my questions. I have a pressurized tank which contains Natural Gas at 200 Bar and 293...

26 February 2021 6,253 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Negative result on natural product sample cholinergic assay?

i have been doing cholinergic assay on natural product and for some of the samples, i get negative value for the inhibition activity. can anyone help me explain the meaning of the negative value...

17 February 2021 4,118 1 View

Can you suggest a good domain identification tool?

I am working on a project to identify sensitive information from text and I am looking into the below categories: -adult content -health -politics -crime Can someone suggest an open source tool...

16 February 2021 4,844 4 View

Prashant Joshi

Better to choose any human neuronal cell line such as PC-12 cell lines.

Becky C. Talyn

There are many indirect (behavioral) assays you could use in Drosophila. Activity, courtship activity, starvation resistance, etc. I'm sure there are also histological & biochemical assays, but that is outside my experience.