What is happening to my multiplex PCR?

I am developing a multiplex PCR assay to identify three strains of bacteria (A, B & C) of which two of them (A & B) are very close-related amongst each other. Two pairs of primers were...

27 May 2020 8,824 6 View

Any article about the effect of optical whitener pretreatment on cotton dyeing process?

I would like someone could share with me any paper about the effect of the pretreament level with optical whitener in the shade of dyed cotton after batch dyeing. I'm specially interested in...

10 September 2018 4,267 3 View

What does a double melting peak in PET DSC Curve mean?

I'm studying two samples of PET, the first is a "virgin" PET (with a white color) and the second is a recycled PET (transparent). In the first run of the first sample there is a double melting...

07 July 2018 9,819 4 View

How to submit 16S rRNA NGS (Illumina MiSeq) data to the public databases?

18 February 2018 556 5 View

I need to express phytase-like protein in some host. Can I use E.coli BL21(DE3) as expression host?It contains phoA and AppA but was used in one paper

I need to express new unknown phytase-like protein in some host to further study its properties (phytase activity, temp. tolerance etc), but have not much experience in protein expression work. I...

09 May 2017 4,895 7 View

Are there an exchange network, social economy in solidarity with family gardens and that contribute to food security?

Could you help me? I need to know about exchange network and social economy solidarity in home gardens. Greetings from Mexico.

13 February 2017 3,566 1 View

No title

I have a 5 years database of 1-minute time basis. I need to calculate the hourly irradiance of a day that represents an entire month of each year . Then I want to compare it. I apply the...

03 November 2016 9,878 1 View

How do I make a 3D cell culture model using A549 cells?

Dear all, i am trying to make a 3D cell culture model using A549 cells, and I have tried several methods such as: - culture in a round bottom plate using methylcellulose, - culture in a...

31 July 2016 4,381 9 View

What is the best programming language for solar radiation data processing?

I just programmed macros in Excel, but I realize that sometimes (when the databases are very large and I would like to calculate many specific variables ) consumes many resources and computing...

29 December 2014 7,401 32 View

What would be the best technique for calibrating a UVER sensor (UVS-E-T) using a UV-B sensor (Solar Light 501 or similar)?

06 December 2014 8,835 2 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Is anyone working on a project focused on immigrants/newcomers working in the hospitality industry and how they have been impacted by COVID?

The on-going Covid-19 pandemic has had devastating economic effects on many industries. However, few have suffered to the same extent as full-service restaurants. Numerous studies have shown that...

24 February 2021 9,496 4 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

What is the role of work experience in motivating high tech entrepreneurship in Aviation industry?

Related work experience plays a vital role in motivating the Entrepreneurship. But when we talk about high tech entrepreneurship in Aviation industry, we talk about very high investment and high...

22 February 2021 376 5 View

Can anyone suggest a topic for the Impact of AI and ML in the financial institutions?

I am currently doing my Master Thesis in FinTech and I am thinking of doing my project on the impact of AI and ML on the financial industry but as the impact of these technologies on the industry...

21 February 2021 7,128 3 View

How do you obtain ASI (Annual Survey of Industries) plant identifiers, to create a panel data?

Dear all, Is there anybody who has worked with ASI (Annual Survey of Industries) Firm-Level data? I have extracted the data files with STATA, and now I need help with creating a panel for...

11 February 2021 876 4 View

Examples of GAMS syntax for a permutation flowshop scheduling problem?

Hi everyone, I am new to the field of industrial engineering. I need to formulate a gams model for a permutation flowshop scheduling problem. I will appreciate if someone can send me a code...

09 February 2021 891 2 View

How does data science impact business?

Data science is valuable to any business in any industry. From statistics and insights across workflows and recruitment and HR to helping senior employees make better-informed decisions, etc. Its...

08 February 2021 5,807 11 View

How to create a Hypothesis and Thesis in a statement?

03 February 2021 4,496 3 View

What's the role of bioinformatics in poultry science?

bioinformatics has a big importance in human science and lots are seeking to study that field, so it attracts me to know it's relation with poultry science and how we can benefit from it in...

20 January 2021 6,963 3 View