A fresh water elliptical unicellular algae was isolated. The DNA was isolated. Amplification with universal primers for 18s rRNA gene did not work, so new primers need to be designed. GC MS analysis of transesterified algal lipids.
The GCMS analysis of the transesterified algal sample is :
Octanoic acid - 1.12%
Tetradecanoic acid - 2.18%
Hexadecanoic acid, methyl ester- 29.94%
9-Octadecenoic acid(Z), methyl ester- 50.66%
9,12-Octadecadienoic acid (Z,Z)-, methyl ester - 16.10%