Since I am working on production of double haploid lines in Onion which requires colchicine as a chromosome doubling chemical, hence please suggest me some more chemicals of similar property as that of Colchicine.
Just curious, aren't you going to use Colchicine? Or, you just need more chemicals for comparing their effect on DH (double haploid)?
Please see this attached paper (2008), on page 31 (highlighted paragraph), it lists some 'doubling agents':
" Freshly isolated and purified microspores were resuspended in NLN liquid medium (Lichter 1985) supplemented with corresponding amounts of particular doubling agent stock solutions to get the final concentrations of oryzalin 1 µmol/l, trifluralin 10 µmol/l and colchicine 50 mg/l (Vyvadilová et al. 1993; Möllers et al. 1994; Hansen & Andersen 1996; Zhou et al. 2002)."
Hello
You can use aqueous solutions of colchicine, trifluralin or oryzalin. In our study, oryzalin was recommended due to its advantages including low toxicity, low cost and its ability to increase ploidy levels at low concentrations.
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