Do salt in PBS affect the TEM image quality ?

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Vladimir Dusevich

Salts form crystals, which may overlay your specimen.

Simone Cantamessa

Good morning,

after fixation you have to wash with water before dehydration. without washes, it may happen to observe samples with saline precipitates

best regards

Nicolas Barois

Hello,

following, the link to a previous discussion about PBS/cacodylate buffer in EM:

https://www.researchgate.net/post/Is_cacodylate_buffer_necessary_in_SEM_protocol_or_I_can_remove_it

I agree with Simone Cantamessa, If you use PBS, you need good washes with water to avoid crystals.