Organoids: An organoid is a three-dimensional organ-bud grown in a laboratory that specializes in regenerative medicine. The technique for growing organoids has rapidly improved since the early 2010s, and it was named by The Scientist as one of the biggest scientific advancements of 2013.[1]
http://en.wikipedia.org/wiki/Organoid
Hi Eduardo,
I have experience with skeletal muscle organoids grown in vitro for 1-2 months. In my opinion the biggest issue is nutrient diffusion along differentiated structure and scaffold maintainance supporting organoid health more than age. With my cell system (C2C12 or feshly isolated mouse satellite cells) encapsualted into a hydrogel matrix (PEG-Fibrinogen) after 2 months the organoid start to soffering mainly for matrix degradation and structure disorganization.
I hope this will help you.
Cesare
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