(1)Do 15NH4Cl and K15NO3 have unique effects different from those of 14NH4Cl and K14NO3 on plants?
(2)Is it practical to use the expensive 15NH4Cl and K15NO3 to improve plant performance under drought conditions?
(3) if we want to examine the effects of 15NH4Cl and K15NO3, parallel study using 14NH4Cl and K14NO3 should have conducted or not.
you use isotope labeling only for tracking nitrogen through metabolic or environmental pathways. Labeled and unlabeled compounds have the same physiological effect in the metabolism. If you want to test different performance of labeled and unlabeled N compounds you are barking up the wrong tree!
In here, isotop labeling will be used to track nitrogen not to improve the plant performance and as far as I know labeled and unlabled compounds have the same effect on plant physiology or metabolism pathways. In a study conducted by Liu et al. (2015, PLOSOne), for calculation of total N contents in different
plant tissue, plants treated with 15NO3-/14NH4+ were combined with plants treated with 14NO3-/15NH4+ because exposure to these differently labelled N sources cannot influence the total N content. So, you don't need to do a parallel study.
Heavy isotopes in N2 reach more slovely than light ones, provided open flows and no charged forms- Steric confrmation is at the base of reaction rate. Your suggestion requires some ground but as far i know the opposite would be probable.
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