In Disc diffusion susceptibility test, on sterile nutrient agar plate broth culture of the test bacteria was swabbed and dried, and 4 sterilized discs, each of 6 mm diameter (prepared in the laboratory from Whatman’s No. 1 filter paper), were placed on 4 different sectors and marked. Thereafter, the discs were soaked with four different concentrations: 20, 35, 50 and 65 μl/disc of ethanolic Bakul Leaf Extracts.
After incubation, ZDIs values obtained around each of the discs were measured. But, clarity of inhibition area lost due to chlorophyll like contents present on the leaf extracts as green tint. In that case, What will be the clarity enhancer procedure without hampering the experimental actuality ?
Try to filtrate the extract through muslin cloth then through filter paper, in this way you will get more transparent extract without other colored contents.
You can use the microbiology scale for measuring the diameter for zone inhibition.
From the figure , you can see the zone of inhibition closer to the disc and the yellow zone is due to the diffusion of extract.
The measured values are c1= 0.6cm in dm; c2=0.5cm; c3= 0.8cm; c4=0.3cm. You can cross check as the zones are visible
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