Does anyone know a colorimetric method that allows you to detect DNA on a microwell plate?
Thanks!
It's pure genomic DNA bound onto the microwell plate. I thought of using probes bound with HRP, but I have no experience with this and also, I would prefer a quantitative method.
Thank you very much for your answer! I also thought of using Hoecht, but came across a second problem: this method should be used in different labs and most of these labs don't have a plate reader which is able to detect fluorescent light...
Hi Veerle
I have not tried out anytime..but see if you can use standard DPA method for colorimetric estimation of DNA present in the microwell plate...
bye
you would have to use of quartz and use the reader havin 360nm wawe;ength if you dont want to use colorometic staining for the same... cheers
Hi David, I have tried to measure absorbance at 260 and plastics indeed do interfere. In my search for a possible solution, I have even tried FlashBlue and FastBlast, but nothing works. I could try to remove the DNA with a detergent but , as you suggested, that would implement the loss of DNA.
Veerle,
Do you need to detect DNA of specific sequences or just gross quantity?
Are you able to liberate the immobilized DNA from the microwell plate?
Hi Leo,
I want to grossly quantify the DNA in the wells, preferably using a colorimetric method. I would opt not to remove the DNA from the wells, due to the loss of DNA, but I could always set up an experiment with liberated DNA.
Colorimetric is insensitive. Many nucleic acid stains (e.g. from invitrogen) also have large absorption coefficients but depending on the quantity of your sample you might not see color in your solution.
What was the problem with FastBlue and FastBlast? non-specific staining or undetectable signal?
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