What is the best method to conduct a meta analysis?

Dear Colleagues: For those of you who have conducted a couple of meta analyses, do you agree with these steps? What is the best method of extracting the data from the chosen studies? Formulate...

26 October 2020 1,539 6 View

Point zero charge Activate carbon, influence of mass?

Hello, I am going to do PZC of some activated biochar/carbon. I thought of doing it by Potentiometric titration with varying pHs and using a salt like NaCl at 0.01M. Now I am wondering: does the...

20 October 2020 5,108 5 View

Is there a database indicating the carbon and nitrogen storage capacity of vascular plants (in Europe)?

Hi everyone, I search a database or a review listing the capacity of vascular plants to storage carbon, nitrogen or other important chemical elements. I work actually on a mountain area (Swiss...

19 October 2020 8,596 3 View

Why is my Tet on system constitutively active and not dox inducible ?

I am using a Tet on system to overexpress a LncRNA in mouse ES cells, based on a piggybac vector (pXlone). I performed transient and stable experiments. In both cases, the LncRNA is expressed even...

13 September 2020 8,303 1 View

With increasing water shortage are the drought resistant plants going to be a solution for food production?

In many areas in the world the lack of water parallels the desertification.Is the creation of new drought resistant plants going to solve the problem for the world nutrition or do we have to...

20 August 2020 8,561 7 View

Do anyone know a good software allowing interactive 3D visualization of density plot?

Hello, I would like to make a interactive 3D representation of some data point. We are studying position of some cell of interest and we extract from serial coronal section XY position of the...

15 June 2020 8,117 2 View

No title

14 June 2020 2,475 7 View

How to extract Pd from Pd(NH3)4Cl2 solution ? Which electrolysis parameters should I choose ?

Hello everybody, I have a solution of Pd(NH3)4Cl2 that I got by refining spent leaching solution of gold. I did many cycles of ammonia (dissolution)/ chlorhydric acid (precipitation) and I got...

23 April 2020 3,048 1 View

How to take into account the SCR when computing the average SCL ?

Hi, I am looking for a suitable method to take into account the non specific SCRs when computing the average SCL. Since non specific SCRs artificially distort the SCL, I want to "remove" them...

30 March 2020 1,625 2 View

Do you think we can trap lymphocytes to manage Lupus crisis?

do you think we can trap lymphocytes to manage Lupus crisis? Something like put a generator of a olecul which attract its and after take of the generator. By this way we could down lymphocyts...

16 January 2020 8,382 6 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Endothelial Functional Assays & Conditions?

Hi everyone, I would greatly appreciate it if someone can shed light on certain endothelial functional assays and their conditions. I often notice that protocols are run with serum-free media, or...

05 February 2021 7,276 1 View

Is there any publication about the effect of basketball and volleyball on body height?

Many people believe that some stretching sports with vertical jump such as basketball and volleyball can increase the body height. On the other hand, others do not agree with this argument. The...

02 February 2021 281 6 View

How can I know the stationary phase of growth of several bacteria?

How can I know the stationary phase of growth of several bacteria? We are aiming to collect exoproteome of several bacteria. We know that the ideal time to collect exoproteome is during the...

31 January 2021 4,528 10 View

No signal/bands observed on western blot for ERK signaling?

My question is regarding a western blot experiment that I am performing to observe pERK signaling by stimulation with VEGF ligand (VEGF165 and VEGF121) on MDAMB231 cells and SW620 cells (both...

30 January 2021 1,039 3 View

How do you annotate figures to show significance?

30 January 2021 1,008 3 View