I need to express the fusion protein purified with GST tag from the BL21 (DE3) host strain for the subsequent protein-protein interaction test. Who can recommend a protein purification kit to me? Thank you very much!
I heard that the purification kit has magnetic bead and non-magnetic bead, which one is more suitable for my experiment?
There are no reall advantages over agarose resins (non-magnetic) to magnetic beads when it comes to the purification of the same tag.
It is mostly a preference in with method you prefer. I personally prefer magnetic beads as I think the setup is pretty easy.
This Youtube Playlist might give you an overview on the different methods here.
https://www.youtube.com/watch?v=ohQKaTeAhzQ&list=PLgKx7UXRO02v5sXoqfl0_czldMa03Pw0g
The examples maybe for His-tagged proteins, but since we are still talking about affinity chromatography here the same principles apply.
On this site here you find GST-tag purification products:
https://cube-biotech.com/products/protein-purification-products/glutathione-gst-tag/
There may be no complete "ready to use"-Kit there, but e.g the MagBeads only need the buffers prepared. (recipes are provided)
I hope this helps you somehow.
https://lnkd.in/eEANJVni
check this video , it may be helpful about GST fusion protein
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