Could you please inform me the crystal structure information of 2-pmmn borophene such as their wyckoff position ?

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What's the optimal plasmid concentration to use when electroporating E.coli DH5alpha?

The volume of E. coli cells to be used is 20 ul.

03 July 2023 4,538 4 View

Is it better to include or exclude the native signal peptide in expression constructs used for the purpose of agroinfiltration?

In effector characterization via agroinfiltration of Nicotiana leaves, I've seen these two options in construct design: constructs with a peptide signal and without. Is there any drawback in...

09 January 2023 1,228 2 View

What statistical test do I use to establish significance between two numbers (not means)?

This is a pre-intervention/post-intervention survey. For each question (total of 21) residents could score a max of 21 points (each question was 0-no, 1-yes). I want to calculate statistical...

12 April 2021 6,784 6 View

Is it possible to quantify recombinant protein in the supernatant using SDS PAGE ?

I would like to quantify a recombinant protein (with native signal sequence) secreted by Pichia pastoris using the SDS PAGE. Since my standard protein (plant origin) is not pure, I would like to...

07 February 2021 4,207 9 View

Which is the best buffer solution to resuspend protein pellet prior to HPLC analysis?

I am working with a sweet-protein 'Thaumatin' (pKa ~ 11) which is secreted by genetically modified Pichia pastoris GS115. Since the protein-producing organism is grown in Basal salts medium, the...

26 October 2020 5,752 3 View

Is there any successful case in the world regarding governance mechanism for coastal island management?

In Bangladesh, Saint Martin's Island is facing threats from natural and anthropogenic stressors. Which governance mechanism could be effective to manage it?

07 April 2020 1,936 0 View

How long do I need to wait to share a paper publicly?

How long do I need to wait to add a paper to RG or any public scholarly domain if I publish without OA? I kind of read something about an embargo period but wasn't clear how long. Could anyone...

15 January 2020 2,414 2 View

Does anyone know a simple and inexpensive lab-scale purification step for biological samples?

Hi, I am currently producing a recombinant protein from yeast Pichia pastoris. The medium used in the bioreactor is the Basal salt medium supplemented with PTM1 salts. I have noticed that when...

19 October 2019 8,599 5 View

How to calculate turbulence intensity and fluctuating triple velocity profile from depth average velocity profile?

I would like to know steps of calculation . So far I know that fluctuating velocities can be found from deference between each streamwise velocity and mean velocity. When I calculate rms value it...

11 April 2019 9,255 14 View

Anyone here who can help me with Insitu hybridization in plants??

I am trying to trace mRNA using Alexa labelled oligo DT probe. There is avery simple protocol to follow in plants however i protocol couldn't get standardized in my hands. Please help me with...

12 August 2018 5,162 0 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Why Powder XRD is performed after cruhsing a crystal if Single Crystal XRD analysis of the same crystal has been performed already ?

What information we can get from PXRD analysis other than from SCXRD analysis of a crystal ?

30 July 2024 6,261 4 View

What should a Mechanical Engineering PhD scholar focus on during their PhD to enhance their chances of securing a postdoctoral position?

29 July 2024 7,714 4 View

May I know the exact Quartile of the journal- Advanced Engineering Materials (Wiley) for material science category?

In some data sources it has been grouped in Q1 and some shows it is Q2.

29 July 2024 4,227 2 View

How to know the b axis of a single crystal?

How to apply magnetic field parallel to b axis of any crystal

29 July 2024 4,083 2 View

Ternary in Electrical Engineering?

Dear Research Community I would like to invite Elctrical Engineering specialists to solve problems related to Ternary Algebra or tripple sets I have an algorithm that places in relation three...

28 July 2024 5,771 2 View

What topic or subject does Production Engineering need to address more?

Contemporary scores or innovations in scientific approaches. Hybrid methodologies, emerging themes and cross-cutting issues?

27 July 2024 648 3 View

How can I use DFT-D3 correction ( Siesta version 5.0.1)?

Dear Siesta Users, I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...

27 July 2024 5,748 0 View

Can a shoot-through event of a tri-state digital buffer cause momentary Hi-Z state?

// interested in the difference between floating events and short circuits.

22 July 2024 6,565 0 View