Could someone recommend an example of pattern recognition in matlab for + signs and detect rotation and misalignment?

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Changming Sun

No code, but this paper could be of interest to you (there is a plus sign, and other shape of signs):

C. Sun and X. Wu, Automatic Segmentation of Fiducial Marks Using Attribute-based Mathematical Morphology. Journal of Electronic Imaging. 10(2):560-566, April 2001.

http://vision-cdc.csiro.au/changs/doc/sun-wu01jei.pdf

Vladimir R. Tuz

The easiest way is to use OpenCV http://opencv.org/

Unfortunately it is not in MatLab but you can use C++, C, Python or Java.