03 March 2022 1 9K Report

Is there an official website or database where I can search the information about forage quality standard? Thank you for your help.

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What will be the possible reasons to cause no bands on gel electrophoresis?

Hi, I am doing RNA extraction for my final year project. After extraction of RNA by using conventional method, I did quantification and qualification of RNA by using Nanodrop spec and gel...

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How to increase the attachment of the HEK293t cells to the glass coverslips or cell dishes?

Hey guys, I always have problems with the attachment of HEK293t cells to glass coverslips while doing IFA. They are so easy to detach from the coverslips while washing. The coverslips are already...

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Can anyone advise me on how to amplify V4 region of fecal DNA of dss-induced colitic mice for microbiota profiling?

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20 December 2020 1,739 3 View

Looking for a glass slide with very low Autofluorescence (GFP)?

Does anyone know any glass slides (thickness 0.1 to 1mm) that have very low autofluoresence (GFP). I am doing a very sensitive experiment that require my glass substrate to be as low fluoresence...

28 November 2020 1,917 6 View

Function of SDS in Northern Blot washing step?

In Northern blot, we normally use either 2*SSC+0.1% SDS or 0.1*SSC+0.1% SDS in washing step. Does anyone know what's the function of the SDS in the washing buffer? Thank you in advance

26 November 2020 2,432 1 View

How to calculate the analytical solution for the HYDRUS-3D model simulation of water and salt movement in soil?

Suppose an agricultural field with uniform soil texture and salinization. The groundwater depth is 20 meters, assuming that the depth of the groundwater and the concentration of salts remain...

25 November 2020 2,382 4 View

Protein concentration decrease after dialysis?

Recently I have been involved in this problem: After expressed by adding IPTG, lysed with B-PER, and resolved with Buffer B (8M urea), incubate with Ni-NTA beads, everything looks fine. When I...

21 November 2020 7,416 3 View

Recommend equipment of in vivo recording in mice brain?

Hello, I am trying to set up an in vivo recording system in mice brain. Which company do you recommend? Neuralynx? TDT? NeuroNexus? Which one do you guys use and why? Please let me know. Thank you!

12 November 2020 2,508 1 View

Looking for a method that aggregate Nano beads on demond?

I am working on a droplet based digital biosensing project. Assume I have some droplets (size 30-40um in diameter), each droplet contians aroun 10-20 Nano beads (100-300nm in diamter). The surface...

28 October 2020 5,761 7 View

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