Controlled cortical impactor devices other than leica?

Michelle Elena Schober @Michelle-Schober

05 September 2023 0 8K Report

We use Leica for controlled cortical impact on 17 day old rats for TBI modelling. Does anyone use another company? I need to replace my current machine.

More Michelle Elena Schober's questions See All

Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

Can I use a likert survey for criterion sampling in a qualitative study or would that make it mixed methods?

I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...

09 February 2021 8,594 3 View

When is it optimal to choose an OFAT (One factor at a time) design over DOE (design of experiment)?

As a current undergraduate student majoring in Microbiology who is still learning DOE (Design of experiment) on my own, I have heard and read that an OFAT design is considered the most inefficient...

29 January 2021 8,256 3 View

Lighting for open field test behavior assay?

I am currently setting up our lab's behavioral space to run the open field test in rats, and I am trying to figure out how to set up lighting to evenly illuminate the open field chamber (a 90 cm x...

26 January 2021 1,399 3 View

Why is my blot transfer bad now?

I had been having beautiful blot transfers, but in the last few days I have ran into issues where there is high background staining and poor band resolution. The background staining is very...

23 December 2020 670 3 View

How/where to provide a dataset?

If someone has collected a large useful dataset that could be useful to social science research, gender research, a specific industry sector in business, and possibly business students or those...

05 October 2020 9,236 6 View

Does amelanotic B16-F10 cells give rise to black toumour nodles when injected in mice?

I am culturing B16-F10 cells in RPMI as these cells tend to die when cultured in DMEM high glucose. When cultured in RPMI, cells are amelanotic. If I inject these amelanotic cells in vivo (i.v.),...

02 September 2020 3,524 1 View

Why do 293T cells starts dying after lentiviral transfection?

I have a lentiviral transfection protocol on 293T cells that has been working for years, but for the past few months, my 293T cells starts de-attaching from the plate. I finally figured out it was...

16 August 2020 6,647 3 View

Any ideas on where to look to for organization theory that speaks to why people join organizations?

I am studying place attachment. Place attachment is usually examined by looking at place and the emotional connection individuals have with place. However, I am interested in learning about how...

11 June 2020 9,483 7 View

What are some of your favorite mixed-methods studies related to environmental management and citizen science/stewardship?

I'd like to learn more about interesting environmental management and citizen science/stewardship studies that combine both quantitative and qualitative methods.

25 May 2020 4,994 1 View

Does the pre-synaptic neuron location in a cortical map influences the spatial position of its synapses in the dendrite of the downstream neuron?

Are functional maps in the cortex used by the brain to carry out computations or are they just a byproduct of wiring minimization? A key element to answer this question is to know if, when...

26 January 2021 1,118 8 View

What are the available certificates for 100% green farms?

Dear All A friend of mine has a farm in Saudi Arabia. He wanted to start installing solar panels to power the two water pumps. It's clear that the solar panels will not work 24/24 but the total...

20 December 2020 2,237 1 View

Using antimicotics such as Nystatin or amphotericin B in mouse neuronal cultures?

Hi all, I just started to prep mouse cortical neutrons for E16.5 and in my second prep I had a massive fungal contamination. Since these preps are quite precious, I wonder if I could add an...

15 September 2020 645 1 View

Extracting surface area, volume and cortical thickness from multiple groups using FreeSurfer?

Hello everyone, I'm new to FreeSurfer and this question might sound a bit stupid: I'm trying to get the volumetric measures, surface area and cortical thickness of brain regions of three groups...

29 July 2020 8,574 3 View

CCT software - brain lesion volume - surface?

I am planning to project a subcortical/cortical brain lesion volume onto the skull surface from a CCT data set. I would be grateful for hints for an appropriate & free software solution for...

17 March 2020 890 4 View

How can I detect cleaved-caspase 3 by western blotting?

Hi everyone, I'm not able to detect cleaved caspase-3 by western blotting using the ab13847 antibody. My wb input are lysed primary cortical neurons treated with staurosporine 1 uM for 4 and 6...

22 September 2019 3,403 3 View

How can I detect cleaved-caspase 3 by western blotting?

Hi everyone, I'm not able to detect cleaved caspase-3 by western blotting using the ab13847 antibody. My wb input are lysed primary cortical neurons treated with staurosporine 1 uM for 4 and 6...

08 September 2019 9,927 4 View

What is the best method for dual transduction with ChR2 and RCaMP1a??

My in-vitro culture consists of E18 rat cortical neurons. I am looking to quantify activity with calcium using GECI, RCaMP1a. The optogenetic experiment relies on the excitation of ChR2 transduced...

08 September 2019 9,598 4 View

Why is it common to use E18 mouse embryos when preparing primary neuronal cultures?

I've been preparing mouse primary neuronal cultures and was wondering if there is a reason many researchers use embryos at stage E18 for this procedure. While cell viability is greater at earlier...

07 August 2019 2,602 5 View

How does one utilise calcium imaged iPSC-derived neurons for immunocytochemistry?

I routinely functionally assess the iPSC derived neurons using calcium imaging with Fluo-4/AM (490nm/510nm). However, have not been successful at using those same neuronal dishes for carrying out...

05 June 2019 3,483 2 View