I have been struggling to obtain the concentration from microplate reader that match up with the Qubit reader. Approximately, they differ by factor of 10.
The Qubit is tolerable for few samples, but if we were to measure for the whole 96 plate, we want to use the microplate reader.
The example of discrepancy is attached in the picture.
The only factor I could think of that may be messing up the values are how I set up the dilution factor series.
Any suggestions/advice would be greatly appreciated!!