CG relaxation with B3LYP in VASP?

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Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

Can we solate viable cells from frozen xenograft tissues?

I am planning to isolate cells from frozen xenograft tissues (stored in liquid nitrogen). The tissues are stored in 10% DMSO, 30%FBS and 60% RPMI with Penn/Strep. These tissues were frozen with a...

14 December 2020 3,910 1 View

How to separate epithelial cells?

Hello everybody, Hope you all are doing fine during this difficult time. I am trying to culture mouse oviductal epithelial cells but, the problem is when I culture them there is a mixture of...

31 October 2020 2,640 4 View

What can you interpret from the estimate of NDVI and LST?

I had estimated the Land surface Temperature and Normalised Difference Vegetation Index, the result shows they have positive relation both increases in lower south part areas and higher in upper...

11 September 2020 6,021 4 View

Hello Everyone, Can anyone help me in the transfection procedure of plasmid into chicken spermatozoa by electroporation?

Hello everyone, I am doing an experiment in which, the plan of work is to electroporate a clone (plasmid form) into chicken spermatozoa, which will be used further for artificial insemination....

07 September 2020 7,855 2 View

Can entropy be reversed? In the Sci-Fi movie TENET, the concept is used to reverse time, is it possible theoretically?

Second Law of Thermodynamics states that as usable energy is lost, chaos increases - and that progression towards disorder can never be reversed.

01 September 2020 2,528 37 View

I am new to cell culture technique and reading papers on cell culture.Is there any criteria for selecting certain cell culture (cell line)?

I need to select a cell type to start my experiment. My experiment is on female genital tract infection and I intend to see cytokine expression. I have read about J774 but I do not know why to use...

09 August 2020 8,504 3 View

What has been going on regarding blood transfusion and heart transplants despite Covid-19?

Heart transplant and blood transfusions are very sensitive whether or not there be any pandemics. How do you think is the pandemic affecting these and how could this be managed, like how blood...

28 May 2020 3,283 9 View

How is the outbreak of Covid-19 affecting handling of other diseases and the management of patients with non-covid cases in your country?

Although Covid-19 has been the massive pandemic, there are still other patients with poor health due to other diseases. Emergence of dengue and kalazar has been noted in some parts of our country....

25 May 2020 9,648 31 View

What is your opinion on virtual learning in school?

My question relates to the context of a developing country such as Nepal. 1. People, in this time of COVID-19 lock- down, are showcasing the application of virtual classes in schools and...

21 April 2020 7,173 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View