Hello, if you have an ability to measure quantity of O2 generated from H2O2 during reaction so one unit of catalase activity is defined as the amount of enzyme required to decompose 1 micromole of H2O2 per minute at pH 7.0 and 25ºC.
If no equipment find triton X method in attachment.
Hello, if you have an ability to measure quantity of O2 generated from H2O2 during reaction so one unit of catalase activity is defined as the amount of enzyme required to decompose 1 micromole of H2O2 per minute at pH 7.0 and 25ºC.
If no equipment find triton X method in attachment.
The signal (change in absorbance) is too small for a reliable calculation. Nevertheless, you would calculate the slope of the absorbance versus time curve after subtracting the background at each time point. This slope would then have to be related to a change in the concentration of peroxide using an extinction coefficient or standard curve.