How can I optimize PCR of 2.3 kb plasmid?

The gene I'm working on is HPPK-DHPS (folate). We isolated it from whole genome of seabuck thorn. So it has introns in it as well. Naturally the primers we designed were are against whole genome...

02 March 2014 9,259 3 View

Would primer designing from a whole genome sequence effect its specificity and performance?

I have a pair of 56 mer Gateway primers for folate gene (HPPK-DHPS). They were designed against folate gene isolated from seabuck thorn. The gene had introns in it as well, so would the presence...

02 March 2014 6,545 8 View

Can any one suggest to me best the method to apply plant extracts in soil? Also how to incorporate antagonistic organisms in soil?

I am currently working on a project where I am supposed to find a biological control for citrus nematode. There are some promising plants with the ability to reduce incidence of citrus nematode in...

01 February 2013 4,301 1 View

The proteins I'm working on are not getting transferred properly - Troubleshooting

I have tried and checked everything. Does someone have an idea what I could have done wrong?

02 March 2012 6,498 16 View

PROJECT DESIGN!

Hey everyone I need some help in my project regarding proteins of CSF in hydrocephalic patients. I am comparing the proteins in CSF of normal and hydrocephalic patients. I wanted to know that...

05 June 2011 4,009 2 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Is receiving Invitation to submit a article from the journal is big deal ???

I have recently received a invitation from the journal "Current Pharmaceutical Bio-technology" to submit a research article. As i didn't received any invitation before. so, is it normal to to...

04 February 2021 4,596 23 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

What steps should one take to address post-harvest disease problems in a supply chain?

If I were to receive an import of a new fruit species in the country and, upon arrival, the whole lot was diseased, what steps do I take to diagnose and solve the problem? Is there a general...

10 January 2021 1,649 7 View

Why negative controls also showing the specific bands of promotor and other regions (GUS, GFP etc.)?

Dear Researchers, I did a transformation of an empty vector (pCAMBIA1301/1302) into my passion fruit plants, for the optimization of the transformation protocol. After transformation; DNA...

06 January 2021 8,465 99 View