How to use less antibody in a Western blot?

I'm trying to figure out how to not need so much antibody for our Western blots. Right now, we use 10 mL of buffer to dilute our antibodies in. Any less than that, and the membrane isn't submerged...

27 August 2019 8,607 3 View

Primer Concentration of RT?

Hello, I am using a gene speciic primer to perform reverse transcription. I need to use 20pmols of my primer in a final reaction volume of 20ul. Is 20pmol interpreted as 20pmol/uL (20uM) in...

28 October 2018 7,946 4 View

What is the relationship between Intelligence and Learning?

Describe the relationship between intelligence and learning? May you please provide journals or articles about this matter. Thanks!

13 June 2018 2,441 8 View

Are there limitations when using AMOS or the PROCESS macro for SPSS to test for moderation?

I've often heard that there is virtually no reason why someone would prefer using AMOS to test for moderation than PROCESS and that it is overall more troublesome. Some even argue that PROCESS is...

01 November 2015 1,282 16 View

How can I compute risk differences and risk ratio estimates from a zero-inflated negative binomial regression for both parts of the model, using SAS?

18 October 2015 9,249 1 View

Wich protocol are you using to stain frozen liver sections with oil red o ?

I have tried till today a lot of protocols (IHC world,...), using isopropanol or propylene glycol or both, but I haven't had any satisfaying results yet...The stain is scattering everywhere out of...

07 September 2015 6,449 7 View

Why do I see "filaments" in my adhesion assays ?

04 March 2015 7,209 2 View

Can someone please help me understand some aspects of conducting a factor analysis please?

I have collected my data and I am now analyzing it. Here are the areas I cannot understand: 1. The pre-post tests questions are alike every way (except in how participants respond after exposure...

18 October 2014 9,540 7 View

Is there any difference in teaching in a University to teaching in a Community College?

I'm being asked if I've ever taught in a Community College as a precursor to a learning/design discussion. There seems to be a belief that teaching and learning in Community Colleges today, are...

30 April 2014 6,878 9 View

What is the best way to induce an s.aureus skin infection in a mouse model?

I'm researching on the effects of plant antimicrobials in S.aureus skin infections, what could be the best way to place the S.aureus?

29 January 2014 9,404 2 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

How to calculate battery consumption for IoT device?

Hi, everyone I'm working on a privacy-preserving algorithm that will apply to IoT devices. So, I need to measure the power consumption by implementing this algorithm. Any suggestion, advice,...

25 February 2021 8,749 6 View

How vulnerable is water and wastewater infrastructure to cyber terrorism in an age of IoT?

Im looking for any papers, reports,articles, links etc on information on How vulnerable is water and wastewater infrastructure to cyber terrorism in an age of IoT.

24 February 2021 2,750 4 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

Open Source IoT Simulators supported by Machine Learning?

Dear Research Gaters, Let me first add context to the question. I am looking to simulate the waste management system and resources of a city. The hardware devices I need to simulate include,...

21 February 2021 2,333 4 View

How to construct different filler shape in MD simulations?

Is there any way to construct different initial filler shapes like triangle, square, rectangle, pillar, and tube in any simulation software.

20 February 2021 1,750 1 View

Where I can find materials to learn Fuzzy VIKOR methodology?

Please suggest ways to learn Fuzzy VIKOR methodology in a best possible way. And is there any toolkit available for the same? Thank you in advance.

20 February 2021 8,271 3 View