Sample size to validate a scale?

I am working on the construction of a new scale measuring protective factors of resilience in teachers. I have a total of 60 items that will be tested (principal component analysis, reliability,...

21 May 2020 2,129 4 View

What image analyzer is best used for CAM assay angiogenesis?

Hi! I am planning to use image J angiogenesis analyzer as my image analyzer for my research study. Our method used is CAM assay. I am still confused on how to use the software. Also, what size of...

10 December 2018 3,786 1 View

Has anyone noticed that Diagenode's Auto iDeal ChIP-seq kit for Histones has a very limited volume of buffers?

01 August 2017 6,263 1 View

How to sequence gut content (plant material) of insect museum specimens?

I'm working on some old dry pinned beetles and am trying to figure out a way to sequence their gut content (plant materials, some may feed on more than one type of plants), what is the best way to...

15 December 2015 1,646 3 View

Can osteogenic and adipogenic differentiation of human MSC be analyzed quantitatively by flow cytometry? If so, can anyone share a protocol?

We would like to induce osteogenesis and adipogenesis in our hMSC lines, but in addition to traditional qualitative staining (i.e. Oil Red O, Alizarin Red, etc), we would like to analyze the...

26 November 2013 1,868 2 View

Online surveys for social sciences researchs: what are your opinions about this tool and how can we improve the research design to incorporate online?

Good morning everyone, Due to the pandemic, I conducted several online surveys. It is quite agile, not at all expensive, but there are several problems: the sample's representativeness and its...

21 February 2021 7,457 3 View

ZnS oxidizes to ZnO!!why??

when I put my ZnS solution in the chemical bath, the ZnS oxidizes so there are ZnO peaks appearing because of the reaction with the oxygen in the air, despite the fact that I close the beaker...

14 February 2021 5,778 9 View

Hi, can a variable in a questionnaire be measured with a series of questions or it has to one question for one variable?

I am doing research on the impact of competitive bidding on project success and would like to use SEM. Project success shall be: cost overage, completion time, quality of works and variation...

13 February 2021 4,413 6 View

Why non-specific band appear on T-ARMS PCR?

Hi everyone. We want to know how can I remove the non-specific band amplification on this variant. Although the amplification of non-specific band is not as intense as the band that we want, but...

09 February 2021 588 2 View

What is your opinion regarding opening of school? Now students are supposed to join school. Do you think that decision is in the interest of society ?

From long Pendamic now government is opening school and colleges on for off line classes. What do you think that this decision is fruitful to the students and society too?

08 February 2021 3,068 6 View

Fractional derivative users, are you on the same page with me on the validity of the fractional order parameter, say alpha?

Taking a fractional ODE problem as an example. It has come to my attention that if the fractional order parameter is in the interval (0,1], many only presents results for alpha=0.9, 0.99. I saw...

08 February 2021 9,671 3 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

Best analysis method to use for my variables?

I need to conduct an analysis and wanted to check if my idea for analysis sounds feasible. I have 3 DVs which are; fear of terrorism, confidence in the police and punitive attitudes towards...

07 February 2021 5,746 10 View

Looking for a scale to measure attitudes towards violence against women and the factors that impact attitudes in South Africa?

I am interested in doing research on attitudes towards violence against women and the factors that impact attitudes in South Africa? Does anyone know which attitude scale I can use to measure...

06 February 2021 3,076 1 View

To what extent do I need to justify my control variables?

Hi everyone, I am writing a research proposal for my masters applications and am in dire need of guidance. I suggest to quantitatively investigate the potential correlation between feminist...

05 February 2021 3,666 8 View